HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III Method Development System1260 Infinity III Amino Acid Analysis System1260 Infinity III Bio-Inert LC System1260 Infinity III LC System1260 Infinity III Prime Online LC System
Price
TypeInquireHPLCBio-Inert HPLCModular HPLC/UHPLCInquire
Detector(s)InquireDAD, FLDInert UV and fluorescence flow cellsVWD, DAD, FLD, RID, ELSDInquire
Flow RateUp to 5 mL/minInquire0.2-10 mL/min0.001-10 mL/min0.001-5 mL/min
Max. Pressure1300 bar600 bar600 bar (8700 psi)600 bar (8700 psi)800 bar
DescriptionUHPLC method development system with automated column and solvent switching, up to 1300 bar.Complete automated HPLC amino acid analysis system.Bio-inert HPLC system for biochromatography with biocompatible flow path.Robust analytical HPLC system with binary, quaternary, or isocratic pump options, up to 600 bar. InfinityLab Assist technology.Online process analysis HPLC system for automated monitoring with real-time data. Quaternary pump, up to 800 bar.
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