HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III High-Throughput System1260 Infinity III Amino Acid Analysis System1260 Infinity III Analytical-Scale LC Purification System1260 Infinity III LC System1260 Infinity III Prime Analytical-Scale LC Purification System
Price
TypeHigh-Throughput UHPLCHPLCInquireModular HPLC/UHPLCInquire
Detector(s)DAD with Max-Light cartridge, LC/MS compatibleDAD, FLDInquireVWD, DAD, FLD, RID, ELSDInquire
Flow Rateup to 5 mL/minInquire0.001-10 mL/min0.001-10 mL/min0.001-5 mL/min
Max. Pressure1300 bar600 bar600 bar600 bar (8700 psi)800 bar
DescriptionHigh-throughput UHPLC system with up to 16 microplates (6144 samples), 1300 bar, 5-second cycle times.Complete automated HPLC amino acid analysis system.Analytical-scale HPLC purification system for preparative work up to 600 bar.Robust analytical HPLC system with binary, quaternary, or isocratic pump options, up to 600 bar. InfinityLab Assist technology.Flexible preparative HPLC/UHPLC system for analytical-scale purification, up to 800 bar.
CapacityUp to 16 cooled microplates (6,144 samples)InquireInquireInquireInquire
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