HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III Method Transfer System1260 Infinity III LC System1260 Infinity III Prime Analytical-Scale LC Purification System1260 Infinity III Prime Bio LC System1290 Infinity III High-Throughput System
Price
TypeMethod Transfer UHPLCModular HPLC/UHPLCInquireBio HPLC/UHPLCHigh-Throughput UHPLC
Detector(s)InquireVWD, DAD, FLD, RID, ELSDInquireBio-Inert VWD, DAD, FLDDAD with Max-Light cartridge, LC/MS compatible
Flow RateUp to 5 mL/min0.001-10 mL/min0.001-5 mL/min0.001-5 mL/minup to 5 mL/min
Max. Pressure1300 bar600 bar (8700 psi)800 bar800 bar (11600 psi)1300 bar
DescriptionUHPLC method transfer system with Intelligent System Emulation Technology, up to 1300 bar.Robust analytical HPLC system with binary, quaternary, or isocratic pump options, up to 600 bar. InfinityLab Assist technology.Flexible preparative HPLC/UHPLC system for analytical-scale purification, up to 800 bar.Versatile bio HPLC/UHPLC system for bioseparations with biocompatible flow path and quaternary pump up to 800 bar.High-throughput UHPLC system with up to 16 microplates (6144 samples), 1300 bar, 5-second cycle times.
CapacityInquireInquireInquireInquireUp to 16 cooled microplates (6,144 samples)
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