HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III HDR-DAD Impurity Analyzer System1260 Infinity III Bio-Inert LC System1260 Infinity III Prime Online LC System1260 Infinity III SFC System1290 Infinity III Bio LC System
Price
TypeInquireBio-Inert HPLCInquireInquireBio UHPLC
Detector(s)HDR-DADInert UV and fluorescence flow cellsInquireInquireVWD, DAD, FLD, Bio MDS, LC/MS
Flow Rateup to 5 mL/min0.2-10 mL/min0.001-5 mL/minUp to 5 mL/minup to 5 mL/min
Max. Pressureup to 1300 bar600 bar (8700 psi)800 bar600 bar1300 bar
DescriptionImpurity analyzer with 30x higher dynamic range and UV sensitivity. UHPLC resolution at up to 1300 bar.Bio-inert HPLC system for biochromatography with biocompatible flow path.Online process analysis HPLC system for automated monitoring with real-time data. Quaternary pump, up to 800 bar.Supercritical fluid chromatography system, up to 600 bar at flow rates up to 5 mL/min.Biocompatible UHPLC system with binary or quaternary options, up to 1300 bar, for biopharma applications.
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