HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III Analytical-Scale LC Purification System1260 Infinity III Prime Bio Analytical-Scale LC Purification System1260 Infinity III Prime Online LC System1290 Infinity III Bio Online LC System1290 Infinity III High-Throughput System
Price
TypeAnalytical-Scale Preparative UHPLCInquireInquireInquireHigh-Throughput UHPLC
Detector(s)DAD (240 Hz), RI, FLD, ELSD, LC/MSD optionalInquireInquireInquireDAD with Max-Light cartridge, LC/MS compatible
Flow Rateup to 5 mL/min0.001-5 mL/min0.001-5 mL/minup to 5 mL/minup to 5 mL/min
Max. Pressure1300 bar800 bar800 bar1300 bar1300 bar
DescriptionAnalytical-scale UHPLC purification system for flow rates up to 5 mL/min, columns up to 10 mm id.Biocompatible HPLC/UHPLC purification system for analytical-scale bio applications, up to 800 bar.Online process analysis HPLC system for automated monitoring with real-time data. Quaternary pump, up to 800 bar.Biocompatible online UHPLC system for real-time bioprocess monitoring with up to 1300 bar.High-throughput UHPLC system with up to 16 microplates (6144 samples), 1300 bar, 5-second cycle times.
CapacityInquireInquireInquireInquireUp to 16 cooled microplates (6,144 samples)
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