HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III Analytical-Scale LC Purification System1260 Infinity III Bio-Inert Analytical-Scale LC Purification System1260 Infinity III LC System1290 Infinity III Bio LC System1290 Infinity III Online SPE System
Price
TypeAnalytical-Scale Preparative UHPLCInquireModular HPLC/UHPLCBio UHPLCInquire
Detector(s)DAD (240 Hz), RI, FLD, ELSD, LC/MSD optionalInquireVWD, DAD, FLD, RID, ELSDVWD, DAD, FLD, Bio MDS, LC/MSInquire
Flow Rateup to 5 mL/min0.001-10 mL/min0.001-10 mL/minup to 5 mL/min0.001-5 mL/min
Max. Pressure1300 bar600 bar600 bar (8700 psi)1300 bar1300 bar
DescriptionAnalytical-scale UHPLC purification system for flow rates up to 5 mL/min, columns up to 10 mm id.Bio-inert HPLC purification system for large biomolecules, up to 600 bar.Robust analytical HPLC system with binary, quaternary, or isocratic pump options, up to 600 bar. InfinityLab Assist technology.Biocompatible UHPLC system with binary or quaternary options, up to 1300 bar, for biopharma applications.Automated online solid phase extraction UHPLC system for analyte enrichment and matrix removal.
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