HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1260 Infinity III Prime Analytical-Scale LC Purification System1260 Infinity III Prime Bio LC System1290 Infinity III 2D-LC System1290 Infinity III Bio LC System1290 Infinity III Online SPE System
Price
TypeInquireBio HPLC/UHPLC2D-LC UHPLCBio UHPLCInquire
Detector(s)InquireBio-Inert VWD, DAD, FLDMultiple detector configurationsVWD, DAD, FLD, Bio MDS, LC/MSInquire
Flow Rate0.001-5 mL/min0.001-5 mL/minup to 5 mL/minup to 5 mL/min0.001-5 mL/min
Max. Pressure800 bar800 bar (11600 psi)1300 bar1300 bar1300 bar
DescriptionFlexible preparative HPLC/UHPLC system for analytical-scale purification, up to 800 bar.Versatile bio HPLC/UHPLC system for bioseparations with biocompatible flow path and quaternary pump up to 800 bar.Two-dimensional LC UHPLC system for comprehensive separations and MS compatibility. Up to 1300 bar.Biocompatible UHPLC system with binary or quaternary options, up to 1300 bar, for biopharma applications.Automated online solid phase extraction UHPLC system for analyte enrichment and matrix removal.
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