HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1260 Infinity III Prime Analytical-Scale LC Purification System1260 Infinity III Multi-Method System1290 Infinity III Bio Analytical-Scale LC Purification System1290 Infinity III Bio LC System1290 Infinity III Method Transfer System
Price
TypeInquireInquireInquireBio UHPLCMethod Transfer UHPLC
Detector(s)InquireInquireInquireVWD, DAD, FLD, Bio MDS, LC/MSInquire
Flow Rate0.001-5 mL/min0.001-10 mL/minup to 5 mL/minup to 5 mL/minUp to 5 mL/min
Max. Pressure800 bar600 bar1300 bar1300 bar1300 bar
DescriptionFlexible preparative HPLC/UHPLC system for analytical-scale purification, up to 800 bar.Multi-method HPLC system for multiple users with automated method switching.Biocompatible UHPLC purification system for multimilligram quantities, up to 1300 bar.Biocompatible UHPLC system with binary or quaternary options, up to 1300 bar, for biopharma applications.UHPLC method transfer system with Intelligent System Emulation Technology, up to 1300 bar.
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