HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1260 Infinity III Bio-Inert Analytical-Scale LC Purification System1290 Infinity III Analytical-Scale LC Purification System1290 Infinity III Bio Analytical-Scale LC Purification System1290 Infinity III HDR-DAD Impurity Analyzer System1290 Infinity III Method Development System
Price
TypeInquireAnalytical-Scale Preparative UHPLCInquireInquireInquire
Detector(s)InquireDAD (240 Hz), RI, FLD, ELSD, LC/MSD optionalInquireHDR-DADInquire
Flow Rate0.001-10 mL/minup to 5 mL/minup to 5 mL/minup to 5 mL/minUp to 5 mL/min
Max. Pressure600 bar1300 bar1300 barup to 1300 bar1300 bar
DescriptionBio-inert HPLC purification system for large biomolecules, up to 600 bar.Analytical-scale UHPLC purification system for flow rates up to 5 mL/min, columns up to 10 mm id.Biocompatible UHPLC purification system for multimilligram quantities, up to 1300 bar.Impurity analyzer with 30x higher dynamic range and UV sensitivity. UHPLC resolution at up to 1300 bar.UHPLC method development system with automated column and solvent switching, up to 1300 bar.
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