Reverse Phase Column / Reversed-Phase HPLC Columns

A reverse phase column, or reversed-phase HPLC columns, are chromatography columns that contain a non-polar stationary phase. Reverse phase columns are widely used in many disciplines, such as pharmaceuticals, the petroleum industry, forensics, and clinical sciences, to separate chemical compounds into their individual parts for purification or analysis. A sample is placed into a reverse phase column and then solvent is added to flush the sample through the stationary phase. Because the stationary phase in a reversed-phase HPLC column is non-polar, the polar components of the sample will drain from the column first, followed by the non-polar components. Reversed-phase HPLC columns can be packed or capillary, made of glass or metal, and can have many different hydrophobic substances as the stationary phase.
CompanyGL SciencesGL SciencesGL SciencesGL Sciences
ItemGL Sciences InertSustain C8GL Sciences InertSustain C18GL Sciences InertSustain PFPGL Sciences InertSustain Phenyl-Hexyl
Price
DescriptionOctyl (C8) bonded reversed-phase column with extreme inertnessUltrapure silica C18 reversed-phase analytical column with evolved surface silica technologyPentafluorophenyl bonded reversed-phase column with unique separation selectivityPhenylhexyl bonded reversed-phase column with complementary selectivity
Mode of SeparationReversed-PhaseReversed-PhaseReversed-PhaseReversed-Phase
Packing MaterialC8C18PFPPhenyl-Hexyl
Pore Size100 Å (10 nm)100 Å (10 nm)100 Å (10 nm)100 Å (10 nm)
Pore VolumeInquire0.85 mL/gInquireInquire
Particle Size2 µm, 3 µm, 5 µm2 µm, 3 µm, 3.5 µm, 5 µm, 10 µm1.9 µm, 3 µm, 5 µm3 µm, 5 µm
Particle Surface Area350 m2/g350 m2/g350 m2/g350 m2/g
End CappingInquireYesYesInquire
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