Reverse Phase Column / Reversed-Phase HPLC Columns

A reverse phase column, or reversed-phase HPLC columns, are chromatography columns that contain a non-polar stationary phase. Reverse phase columns are widely used in many disciplines, such as pharmaceuticals, the petroleum industry, forensics, and clinical sciences, to separate chemical compounds into their individual parts for purification or analysis. A sample is placed into a reverse phase column and then solvent is added to flush the sample through the stationary phase. Because the stationary phase in a reversed-phase HPLC column is non-polar, the polar components of the sample will drain from the column first, followed by the non-polar components. Reversed-phase HPLC columns can be packed or capillary, made of glass or metal, and can have many different hydrophobic substances as the stationary phase.