| Description | 4titude 190μm clear base imaging microplate, black frame. Optically Clear Tissue Culture Plates have been designed for high content screening (HCS) assays in drug development and related areas. They are also suitable for homogeneous assays, employing fluorescence intensity, FRET and TR-FRET 4titude 190μm clear base imaging microplate, black frame. Optically Clear Tissue Culture Plates have been designed for high content screening (HCS) assays in drug development and related areas. They are also suitable for homogeneous assays, employing fluorescence intensity, FRET and TR-FRET where measurements are bottom-read. The high-quality optical base plate assures the necessary accuracy and consistency for automated high throughput systems, generating optimum signal-to-noise ratios. Using state-of-the-art manufacturing technology, we have developed a product which offers several key advantages to the end user. Optically Clear Tissue Culture Plates are assembled using unique patented laser welding technology which reduces auto fluorescence and does not inhibit cell growth. Available with 190μm polystyrene base. Available TC treated: our advanced tissue-culture (TC) treatment method evenly coats each well for optimal cell adhesion properties for highest reproducibility between wells, plates and batches. Key Features: - Leak free
- Free from DNase, RNase, human genomic DNA, and cyto-toxic
- Optimum signal-to-noise ratios
- Good cell adhesion
- Wicking and bubble formation eliminated
- SBS footprint
- Alphanumeric grid reference
- Recommended for confocal microscopy due to reduced cross-talk and superior base flatness
- Reduced auto fluorescence ensures their suitability for fluorescent assays
- Suitable for assays that measure absorbance in the visible light range (400-900nm wavelengths)
- Suitable for colorimetric assays
- Suitable for homogeneous assays
- Suitable for use in BMG lab tech, Molecular Devices, Promega Glomax and other plate readers
- Suitable for adhesive and heat sealing: our Moisture Barrier Seal 96 (4ti-0516/96) is recommended as it is optically clear and allows repeated imaging without removal, reducing contamination risks
- Available with 190μm polystyrene base
- All plates come sterilized, apart from 4ti-0224
- TC treated option available (4ti-0221)
- Available barcoded upon request
... Read More | 4titude ultra-clear base imaging microplate. The main advantage of using Ultra Optically Clear Plates is in the optically ultra-clear base of the plate, which gives superior results by delivering low absorbance and high transmission, together with low background signals. Not only this, but due to 4titude ultra-clear base imaging microplate. The main advantage of using Ultra Optically Clear Plates is in the optically ultra-clear base of the plate, which gives superior results by delivering low absorbance and high transmission, together with low background signals. Not only this, but due to its incredible optical clarity, the ultra-clear base of these plates delivers improved transmission of signals at low wavelengths compared to standard optical films. It allows DNA measurements at 260/280nm wavelengths in a medium or high throughput. Key Features: - Free from DNase, RNase and human genomic DNA
- Ultra-clear base improves transmission for low wavelengths
- Optimum signal-to-noise ratios
- Alphanumeric grid reference to aid well and sample identification
- Suitable for adhesive and heat sealing
- Available barcoded upon request
- Lid available (4ti-0290)
... Read More | 4titude standard profile, 0.2ml polypropylene wells, polycarbonate frame with upstand, cut corner A12, working volume: <200μl, total well capacity: 300μl; designed for use on ABI® instruments. This ABI® Style PCR plate offers the benefits of our 2-component design to ABI® 4titude standard profile, 0.2ml polypropylene wells, polycarbonate frame with upstand, cut corner A12, working volume: <200μl, total well capacity: 300μl; designed for use on ABI® instruments. This ABI® Style PCR plate offers the benefits of our 2-component design to ABI® standard block users. This design combines the advantages of ultra-thin wall polypropylene tubes for optimum PCR results with a rigid polycarbonate skirt and deck for the highest thermal stability. We recommend this semi-skirted plate for use with ABI® thermal cyclers and sequencers; it can be used directly with ABI® instruments with no adapters necessary. The only case where this is not true is with the ABI Fast Block thermal cyclers; in this case, using our FrameStar Fast Plate is recommended instead. Key Features: - Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time quantitative PCR (RT-qPCR) results
- <0.2ml (200μl) working volume, 0.3ml (300μl) total well capacity
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
- Ideal for use with ABI® thermal cyclers & sequencers
- Recommended for low volume PCR
- Compatible with standard multichannel pipettes
- Ideal for use with robotic systems
- Available with the following frame color options for the clear well variety: purple, blue, clear, green, red, and black
- Ethylene oxide treated option available for forensic use
- Available barcoded upon request
- Combi packs available (for 4ti-0730/C) with Optically Clear Windowed qPCR Seal (4ti-0565)
... Read More | 4titude low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100μl, total well capacity: 200μl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays. Our 4titude low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100μl, total well capacity: 200μl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays. Our FrameStar Roche Style plates are designed to achieve optimized assay conditions on the Roche LightCycler® 96 and 480 (with 96 well block). This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100μl. The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20μl. The extra white wells allow for increased sensitivity in fluorescent assays. The semi-skirted frame allows for labeling or barcoding. Key Features: - Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- <0.1ml (100μl) working volume, 0.2ml (200μl) total well capacity
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
- Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
- Recommended for low volume PCR
- Compatible with standard multichannel pipettes
- Ideal for use with robotic systems
- Plates are available with a clear polycarbonate frame and extra white wells for improved sensitivity when using fluorescent based assays
- Combi packs available with qPCR Seal (4ti-0560)
- Available barcoded upon request
... Read More | 4titude peelable heat sealing foil with wide material compatibility; suitable for low temperature storage, high temperature uses and PCR Heat sealing offers a 100% effective method of plate sealing, for complete seal integrity, as well as being quick and cost effective. Our Universal Peel Heat Seal 4titude peelable heat sealing foil with wide material compatibility; suitable for low temperature storage, high temperature uses and PCR Heat sealing offers a 100% effective method of plate sealing, for complete seal integrity, as well as being quick and cost effective. Our Universal Peel Heat Seal is a laminate seal compatible with PP, PE, PS, COC and PC plates, providing the highest flexibility in plate material choice. Sample access is possible by peeling from the compatible materials and also by piercing the seal with needles, but not with plastic tips. It is resealable by applying another Universal Peel Heat Seal directly on top of a previously pierced seal. Universal Peel Heat Seal forms a complete seal to a plate enabling low temperature uses as well as high temperature uses, such as PCR (when used with a pressurized heated lid). The seal demonstrates moderate solvent resistance. Use of Universal Peel Heat Seal makes roll changes unnecessary even for customers using different microplate materials, because with minor adjustments of sealing parameters all common microplate materials can be sealed to. This seal is available as sheets, for use with manual and semi-automated sealers, such as our Semi-Automated Sheet Heat Sealer. Also available in multiple roll formats compatible with specified automated heat sealers, such as our Automated Roll Heat Sealer. Key Features: - Pierceable with needle, but not with standard pipette tips
- Peelable
- Seal integrity temperature range: -80°C to 90°C (110°C when used with a pressurized heated lid)
- Autoclavable (121°C)
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
- Suitable for low temperature sample storage and high temperature uses, such as PCR
- Compatible with PP, PE, PS, COC and PC plates
- Wide material compatibility allows for high throughput sealing of different plates without the need for roll changes
- Resealable by applying another Universal Peel Heat Seal directly on top of a previously pierced seal
- Sheet format: 125mm x 78mm, to fit all standard SBS footprint PCR and qPCR plates, microplates, assay and storage plates, from 12 well to 1536 well
- Roll format: available in two roll dimensions, to suit your choice of automatic heat sealing equipment
... Read More |