| Description | Anti Iba-1 is specifically reactive to microglia and macrophages, and are appropriate for immuno-double staining of brain tissues and cell culture in combination with monoclonal antibody to GFAP, which specifically reactes to astrocytes. | monoclonal antibody, Clone 1E6 | Immunoglobulin fractions: IgG, IgA, and IgM. Each package comes with in vivo and in vitro titration data for the lot.
PREPARATION:Repeated immunization was performed with purified Asialo GM1 from bovine brain tissue, in conjunction with methylated bovine serum albumin and complete Freund's adjuvant.Immunoglobulin fractions: IgG, IgA, and IgM. Each package comes with in vivo and in vitro titration data for the lot.
PREPARATION:Repeated immunization was performed with purified Asialo GM1 from bovine brain tissue, in conjunction with methylated bovine serum albumin and complete Freund's adjuvant. Gammaglobulin fraction of serum was obtained by 50% ammonium sulfate precipitation methods followed by dialysis with phosphate buffered saline (pH 7.2)
SPECIFICITIES: Reacts with mouse and rat NK cells; mouse monocytes (liver cells which contain no NK cells; bone marrow; fetal liver cells; spleen cells of nude mice-macrophages); fetal thymocytes (12 days old; ratio of existence decreased gradually until there were none in newborn mice). Anti Asialo GM1 antiserum has been shown to eliminate natural killer (NK) activity in cells of various strains of mice and rats.... Read More | 'Preparation: Lyophilized, salt-free
Specificity: In ammouniun carbonate buffer (pH 7.8 or ammonium acetate buffer (pH 4.0): Cleaves C-terminal peptide bonds of glutamic acid. In phosphate buffer (pH 7.8): Cleaves C-terminal peptide bonds of glutamic acid and aspartic acid.
Pkg: 50 µg | This ligase will be used for ligation of ssDNA-ssDNA, ssRNA-ssRNA and ssDNA-ssRNA depending on ATP. Reaction of this ligase in 55-65 degrees, which is the optimum reaction temperature, will promote the ligation efficiency. |