| Description | Product Characteristics The Synthetic Blocking Buffer, Blotting is a readyto-use solution for blocking of the remaining free binding sites after coating or transfer of biomolecules to a membrane. The buffer blocks all potential binding sites of nonspecific interaction and reduces the background Product Characteristics The Synthetic Blocking Buffer, Blotting is a readyto-use solution for blocking of the remaining free binding sites after coating or transfer of biomolecules to a membrane. The buffer blocks all potential binding sites of nonspecific interaction and reduces the background signal. The Synthetic Blocking Buffer, Blotting eliminates the risk of false positive reactions in the assay, while avoiding the current safety hazards related to the use of bovine biological material, for instance BSAComposition & Properties The Synthetic Blocking Buffer, Blotting is a readyto-use buffer. The buffer contains no proteins, and cross reactivity is therefore avoided.Working Procedure 1.Follow the normal procedure for transfer of the biomolecules to the membrane. 2.Dry the membrane. 3.Block the membrane by incubation in the Synthetic Blocking Buffer, Blotting for 5-10 minutes at room temperature and gentle shaking. 4.Wash the membrane in a standard washing buffer for blotting. 5. Continue the processing as usual.Tips & Tricks • In ELISA, the Synthetic Blocking Buffer, ELISA (cat. no. S494401) is recommended.Handling & Storage • Store solution at 2-8 °C... Read More | Mammalian lactate dehydrogenases (LDH) exist as five tetrameric isozymes composed of combinations of two different subunits. The H subunit predominates in heart muscle, which is geared for aerobic oxidation of pyruvate. The M subunit predominates in skeletal muscle and is concerned more with Mammalian lactate dehydrogenases (LDH) exist as five tetrameric isozymes composed of combinations of two different subunits. The H subunit predominates in heart muscle, which is geared for aerobic oxidation of pyruvate. The M subunit predominates in skeletal muscle and is concerned more with anaerobic metabolism and pyruvate reduction.Catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+Recombinant rabbit muscle Lactate Dehydrogenase produced in E.Coli. Chromatographically purified. A lyophilized powder... Read More | Inquire | Purity>95% SDS-PAGEFunctionHas hepatocyte mitogenic activity | Purity≥ 92% SDS-PAGEActual molecular weight 15&17kDaFunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like Purity≥ 92% SDS-PAGEActual molecular weight 15&17kDaFunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis... Read More |