| Description | Protein Accession Number: P00330CATH Classification (v. 3.2.0):Class: Alpha BetaArchitecture: Alpha-Beta Complex and 3-Layer (aba) SandwichTopology: Quinone Oxidoreductase; Chain A, domain 1 and Rossmann foldMolecular Weight: 146.8 kDa (Theoretical)Optimal pH: 5.4 (Shore and Theorell 1966)Protein Accession Number: P00330CATH Classification (v. 3.2.0):Class: Alpha BetaArchitecture: Alpha-Beta Complex and 3-Layer (aba) SandwichTopology: Quinone Oxidoreductase; Chain A, domain 1 and Rossmann foldMolecular Weight: 146.8 kDa (Theoretical)Optimal pH: 5.4 (Shore and Theorell 1966)Isoelectric Point: 6.23 (Theoretical)Extinction Coefficient:189,320 cm-1 M-1 (Theoretical)E1%,280 = 12.89 (Theoretical)Activators:Sulfhydryl activating reagentsMercaptoethanolDithiothreitolCysteineHeavy metal chelating agents(White and White 1997)Inhibitors:Heavy metals and -SH reagentsThioureaPurine and pyrimidine derivativesChloroethanol and fluoroethanolN-alkylmaleimidesIodoacetamide1,10-phenanthroline8-hydroxyquinolineBeta-NAD analogs (White and White 1997)Applications:Enzymatic determination of primary alcohols, and aldehydesSynthesis of chiral compoundsSpectrophotometric assay of plasmalogenaseEnzymatic catalysis in organic solventsStudies of NAD+, NADH, NADP+, and NADPH... Read More | Inquire | Inquire | Lipoprotein Lipase Activator is a cell-permeable benzylphosphonate derivative that selectively induces lipoprotein lipase (LPL) mRNA and protein levels, but does not exhibit PPARα or PPARγ agonistic activities. Lipoprotein Lipase Activator lowers serum lipid levels and plasma triglyceridesLipoprotein Lipase Activator is a cell-permeable benzylphosphonate derivative that selectively induces lipoprotein lipase (LPL) mRNA and protein levels, but does not exhibit PPARα or PPARγ agonistic activities. Lipoprotein Lipase Activator lowers serum lipid levels and plasma triglycerides with concomitant elevation in high-density lipoprotein cholesterol (HDL-C) in animal models. Lipoprotein Lipase Activator also induces fatty acid oxidation related enzymes, lowers free fatty acids (FFA), and minimizes fat accumulation. Also reported to suppress the plasma levels of TNF-a and COX-2 and displays anti-tumor properties... Read More | Purity≥95% SDS-PAGE. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.FunctionPro-inflammatory cytokine. Involved in the innate immune response to Purity≥95% SDS-PAGE. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.FunctionPro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity... Read More |