| Description | Product usage adviceIncubation temperature: 20-37 °C / 68-100 °FResponse time: Moderate (up to 30 minutes) at 20-37°CShelf life: 24 monthsActive ingredients and principle of actionIn different buffer solutions (pH = 9.5), different additives were added as required, and the active Product usage adviceIncubation temperature: 20-37 °C / 68-100 °FResponse time: Moderate (up to 30 minutes) at 20-37°CShelf life: 24 monthsActive ingredients and principle of actionIn different buffer solutions (pH = 9.5), different additives were added as required, and the active ingredient p-nitrophenyl phosphate (pNPP) was dissolved.Alkaline phosphatase transfers phosphate residues to acceptors. Under alkaline conditions, a yellow color will appear due to the formation of nitrophenols.Main advantageHigh absorbance yield comparable to freshly dissolved pNPP tabletsWide measuring rangelow background signalLow blank drift during long-term storage (<0.18 AU over 24 months)High color stability after quenching the reaction with used stop solutionStorage, Shipping and Filling InformationStore at 2-8°C protected from light. Under these conditions, the expiration date printed on the label is valid for unopened bottles.Spontaneous decay increases the background. The rate of decay increases if stored at room temperature. Therefore, storage and transport at room temperature should be avoided. However, the activity of the solution was not affected by storage at room temperature. The solution remains valid beyond the due date, but some applications, especially those that include visual assessments, may be hindered by increased background.Contaminated or leaked substrate solutions from damaged bottles should not be reused and must be destroyed.Ship in an isolated container with some refrigerated bags.The following bottling instructions should be considered:Work in a dust-free and dark room.Keep the solution as cool as possible.Avoid contact of solution with any metal parts.Thoroughly clean all instruments and containers.Wear powder-free gloves when bottling.Close the bottle immediately to minimize the effects of light and dust.Use an opaque, clean bottle made of HDPE or PP.General instructions for use in ELISAOnly qualified laboratory staff familiar with the basics of immunological methods should use these solutions.Substrate solutions can be used in qualitative and quantitative ELISA procedures.When using a 96-well microtiter plate, it is recommended to add 100 µL of pure substrate per well after incubation and washing. After substrate incubation, the reaction can be stopped and photometric measurements can be performed. Using a higher incubation temperature (37°C) may shorten the incubation time.stop 2. Further increases in signal cannot be safely ruled out using other commercially available stopping solutions. The addition of stop solution did not change the general shape of the spectrum.Unstopped and stopped solutions should be measured at 405 nm, background corrected: should be measured at 620 nm.WasteDisposal of waste must comply with national and local laws and regulations. Disposal of packaging must comply with national and local disposal regulations... Read More | Product introduction：The additive contains a variety of cytokines, human albumin and other components. In order to ensure the activity, it is recommended that the additive part be thawed once and then sub packaged and frozen again. It is not advisable to freeze and thaw repeatedly. The Product introduction：The additive contains a variety of cytokines, human albumin and other components. In order to ensure the activity, it is recommended that the additive part be thawed once and then sub packaged and frozen again. It is not advisable to freeze and thaw repeatedly. The product contains no animal serum or animal serum components, no animal protein components, and no antibiotics.Matters needing attention：1. try to reduce the number of repeated freezing and thawing to avoid efficiency decline. 2. it is not suitable to place it at room temperature for a long time. 3. pay attention to aseptic operation and try to avoid pollution. 4. please wear experimental clothes and disposable gloves for operationScope of application：Cell culture additives... Read More | IRE1α kinase-IN-2 is a potent IRE1α kinase inhibitor, with an EC 50 of 0.82 µM. IRE1α kinase-IN-2 inhibits IRE1α kinase autophosphorylation (IC 50 =3.12 µM). IRE1α kinase-IN-2 inhibits XBP1 mRNA splicing in the WT cell lines.In VitroIRE1α kinase-IN-2 (compoundIRE1α kinase-IN-2 is a potent IRE1α kinase inhibitor, with an EC 50 of 0.82 µM. IRE1α kinase-IN-2 inhibits IRE1α kinase autophosphorylation (IC 50 =3.12 µM). IRE1α kinase-IN-2 inhibits XBP1 mRNA splicing in the WT cell lines.In VitroIRE1α kinase-IN-2 (compound 3) inhibits XBP1 mRNA splicing, even during ER stress. MCE has not independently confirmed the accuracy of these methods. They are for reference only.Form:Solid... Read More | Inquire | Inquire |