| Description | Products contentNote: The 10 x PCR Buffer for this product contains 15 mM magnesium ions. Products IntroductionFastPrime DNA Polymerase is a mixture of anti-Taq enzyme monoclonal antibody and Taq DNA Polymerase with high amplification and fidelity properties for HOT Start PCR. When using FastPrime Products contentNote: The 10 x PCR Buffer for this product contains 15 mM magnesium ions. Products IntroductionFastPrime DNA Polymerase is a mixture of anti-Taq enzyme monoclonal antibody and Taq DNA Polymerase with high amplification and fidelity properties for HOT Start PCR. When using FastPrime DNA Polymerase for PCR amplification, Taq enzyme antibody binds to Taq enzyme and inhibits DNA polymerase activity before denaturation at low temperature. When using FastPrime DNA Polymerase for PCR amplification, before denaturation at high temperature, Taqase antibody binds to Taqase to inhibit DNA polymerase activity, which can effectively inhibit the non-specific annealing of primers and non-specific amplification caused by primer dimer at low temperature, and the Taqase antibody denatures during the initial DNA denaturation step of the PCR reaction, and the activity of DNA polymerase is restored, thus achieving the effect of Hot Start. No special inactivation of the Taqase antibody is required for the use of this product, and it can be used under conventional PCR reaction conditions.FastPrime DNA Polymerase has 5′→3′ DNA polymerase activity, 5′→3′ exonuclease and 3′→5′ exonuclease activity. Compared with Taq DNA Polymerase, FastPrime DNA Polymerase has high amplification efficiency and low mismatch rate, and can amplify DNA fragments efficiently. The PCR products amplified with this product have an "A" base at the 3′ end, which can be directly used for T/A cloning. This product is suitable for routine PCR reactions and gene cloning reactions that require high fidelity.Definition of activityThe amount of enzyme required to dope 10 nmol of deoxyribonucleotide into acid-insoluble material was defined as 1 activity unit (U) at 74°C for 30 min, using activated salmon sperm DNA as template/primer.quality control After several column purification, its purity is more than 99% by SDS-PAGE; no exogenous nuclease activity is detected; no host residual DNA is detected by PCR method; it can effectively amplify single-copy genes in the human genome.UsageThe following is an example of a PCR reaction system and reaction conditions for amplifying a 1 kb fragment of human genomic DNA as a template, which should be improved and optimized according to the template, primer structure and size of the target fragment in actual operation.1. PCR reaction systemNote: Please use the final concentration of 0.1-1.0 µM as a reference for setting the range of primer concentration. If the amplification efficiency is not high, the primer concentration can be increased; if a non-specific reaction occurs, the primer concentration can be decreased to optimize the reaction system. 2.PCR reaction conditionsAttention: 1）In general, the annealing temperature is 5°C lower than the melting temperature of the amplification primer, Tm. When the desired amplification efficiency cannot be obtained, the annealing temperature should be lowered appropriately; when non-specific reaction occurs, the annealing temperature should be increased, so as to optimize the reaction conditions.2）The extension time should be set according to the size of the amplified fragment, and the amplification efficiency of this product is 2 kb/min. 3) The number of cycles can be set according to the downstream application of the amplified product. If the number of cycles is too low, the amount of amplification will be insufficient; if the number of cycles is too high, the chance of mismatch will increase and the non-specific background will be serious. Therefore, the number of cycles should be minimized under the premise of ensuring the product yield... Read More | Product DescriptionEndo F2 cleaves N-linked (asparagine-linked) biantennary oligosaccharides from glycoproteins. It also will cleave high mannose glycans but at a 40x reduced rate. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, Product DescriptionEndo F2 cleaves N-linked (asparagine-linked) biantennary oligosaccharides from glycoproteins. It also will cleave high mannose glycans but at a 40x reduced rate. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact.Endoglycosidase F2 is less sensitive to protein conformation than PNGase F and is therefore more suitable for deglycosylation of native proteins. However, for optimal results, denaturation of the glycoprotein is recommended.Contents60 µl aliquot of enzyme (0.3 U) in 10 mM sodium acetate 25mM NaCl, pH 4.5Included with 20 µL and 60 µL pack sizes:5x Reaction Buffer – 250 mM sodium acetate, pH 4.5Molecular weight 32,000 daltonsSpecific Activity Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of denatured porcine fibrinogen in 1 minute at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE (cleaved fibrinogen migrates faster).Formulation The enzyme is provided as a sterile-filtered solution in 10 mM sodium acetate, 25mM NaCl, pH 4.5Specificity Endo F2 cleaves Asparagine-linked biantennary and high mannose glycans (at a 40X reduced rate). It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact. Endoglycosidase F2 is less sensitive to protein conformation than PNGase F and is therefore more suitable for deglycosylation of native proteins. However for optimal results, denaturation of the glycoprotein is recommended.Quality & Purity Endo F2 is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated at 37°C for 24 hours with 2 µl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation. The production host strain has been extensively tested and does not produce any detectable glycosidases.Stability Several days exposure to ambient temperatures will not reduce activity. Stable at least 12 months when stored properly.Directions for use 1. Add up to 200 µg of glycoprotein to an Eppendorf tube. Adjust to 38 µl final volume with de-ionized water. 2. Add 10 µl 5x Reaction Buffer 4.5 3. Add 2.0 µl of Endo F2 to the reaction. Incubate 1 hour at 37°C. Monitor cleavage by SDS-PAGEThe production host strain has been extensively tested and does not produce any detectable glycosidases... Read More | Gly-Pro-pNA hydrochloride is a dipeptidyl peptidase inhibitor that inhibits dipeptidyl peptidase II, dipeptidyl peptidase IV and dipeptidyl peptidase IX | Purity>95% (SDS-PAGE) Endotoxin level<1.0 EU/µgFunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells | Purity>97% by SDS-PAGE and HPLC analyses.Additional sequence informationN-terminal Glycine.FunctionChemotactic for monocytes and T-lymphocytes. Binds to CXCR3.Post-translationalCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes |