| Description | GoldStar DNA Polymerase is a chemically modified and highly efficient Taq DNA Polymerase. The enzyme's activity is completely blocked at room temperature, making it inactive at low or room temperature. This effectively avoids non-specific amplification caused by non-specific binding of primers and GoldStar DNA Polymerase is a chemically modified and highly efficient Taq DNA Polymerase. The enzyme's activity is completely blocked at room temperature, making it inactive at low or room temperature. This effectively avoids non-specific amplification caused by non-specific binding of primers and templates or primer dimers at room temperature. Enzyme activation requires incubation at 95 ℃ for 10 minutes. The unique buffer system makes the application of enzymes more extensive, enabling efficient amplification of high GC content, complex secondary structures, and low copy templates. Using this product for PCR amplification, the 3 'end of the PCR product contains an "A" base, which can be directly used for T/A cloning. This product has strong specificity and does not require gel recovery to remove impurities after PCR amplification. It can be directly used for downstream cloning or chip hybridization experiments. Mainly used for routine PCR, RT-PCR, Real time PCR, multiplex PCR, gene chip analysis, and SNP detection, especially suitable for PCR reactions that require high specificity.G665840Component500 U2500 UStorageG665840AGoldStar DNA Polymerase, 5 U/µL100 µL 5×100 µL-20℃. Avoid freeze/thaw cycle.G665840B5×GoldStar PCR Buffer1.9 mL 5×1.9 mL-20℃. Avoid freeze/thaw cycle. Attention: The 5 x GoldStar Taq PCR Buffer of this product contains 8.5mM magnesium ions.Unit DefinitionUsing activated salmon sperm DNA as a template/primer, the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into acidic insoluble substances is defined as 1 active unit (U) at 74 ℃ for 30 minutes.Quality controlSDS-PAGE detection shows a purity greater than 99%; No exogenous nuclease activity detected; PCR method for detecting host free residual DNA; Can effectively amplify single copy genes in the human genome. Store at room temperature for one month without significant changes in activity.UsageThe following is an example of a PCR reaction system and reaction conditions for amplifying a 1 kb fragment using human genomic DNA as a template. In practical operation, corresponding improvements and optimizations should be made based on the template, primer structure, and target fragment size.1. PCR reaction system Reagent 50 µl Reaction system Final concentration 5×GoldStar PCR Buffer 10 µl 1 × dNTP Mix，10 mM each 1 µl 200 µM each Forward Primer，10 µM 2 µl 0.4 µM Reverse Primer，10 µM 2 µl 0.4 µM Template DNA <0.5 µg <0.5 µg/50 µl GoldStar DNA Polymerase，5 U/µl 0.5 µl 2.5 U/50 µl ddH2O up to 50 µl / Attention: Primer concentration, please use final concentration of 0.1-1.0 µ M serves as a reference for setting the range. In the case of low amplification efficiency, the concentration of primers can be increased; When non-specific reactions occur, the primer concentration can be reduced to optimize the reaction system.2. PCR reaction conditions Step Temperature Time / Pre denaturation 95℃ 10 min / Denaturation 95℃ 30 s 30-40 cycles Annealing 55-65℃ 30 s 30-40 cycles Extension 72℃ 60 s 30-40 cycles Final extension 72℃ 5 min / Attention:1) In general experiments, the annealing temperature is 5 ℃ lower than the melting temperature Tm of the amplification primer, and the annealing time is generally 30-60 seconds. If the ideal amplification efficiency cannot be achieved, the annealing temperature should be appropriately reduced; When non-specific reactions occur, increase the annealing temperature to optimize the reaction conditions.2) The extension time should be set according to the size of the amplified fragment. The amplification efficiency of GoldStar Taq DNA Polymerase contained in this product is 1-2 kb/min.3) The number of cycles can be set based on the downstream application of the amplification product. If the number of cycles is too small, the amplification amount is insufficient; If there are too many cycles, the probability of mismatches will increase, and non-specific backgrounds will be severe. So, while ensuring product yield, the number of cycles should be minimized as much as possible.4) This product must achieve enzyme activation under pre denaturation conditions of 95 ℃ and 10 minutes... Read More | p53 and MDM2 proteins-interaction-inhibitor dihydrochloride is an inhibitor of the interaction between p53 and MDM2 proteins.Form:Solid | Inquire | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. 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Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator... Read More | VEGF permeability factor, also known as Vascular permeability factor (VPF), is a highly specific permeability factor for endothelial growth factor. It can promote the increase of vascular permeability, extracellular matrix degeneration, vascular endothelial cell migration, proliferation and VEGF permeability factor, also known as Vascular permeability factor (VPF), is a highly specific permeability factor for endothelial growth factor. It can promote the increase of vascular permeability, extracellular matrix degeneration, vascular endothelial cell migration, proliferation and angiogenesis. VEGF has also been shown to have chemotaxis on monocytes and osteoblasts.OsrhVEGF is expressed by oryza sativa and purified by protein purification technology... Read More |