| Description | Product Descriptionalpha-L-fucoside fucohydrolase, alpha-L-fucosidase, alpha-(1-6) fucosidaseRemoves non-reducing, terminal branched fucose when linked alpha-(1-6) to the core GlcNAc of a GlcNAc-GlcNAc disaccharide structure that has been labeled with a fluorescent molecule. To date, the fluorescentProduct Descriptionalpha-L-fucoside fucohydrolase, alpha-L-fucosidase, alpha-(1-6) fucosidaseRemoves non-reducing, terminal branched fucose when linked alpha-(1-6) to the core GlcNAc of a GlcNAc-GlcNAc disaccharide structure that has been labeled with a fluorescent molecule. To date, the fluorescent labels ANTS, ANDSA and 2-AA have been demonstrated to function with the fucosidase.There is no cleavage of unlabeled oligosaccharides.The one exception is that a terminal unbranched α(1-3) or α(1-4) fucose is cleaved in the absence of any reporter molecule.α(1-6) Fucosidase is useful for determining core fucosylation.ContentsAlpha-(1-6) fucosidase in 20 mM Tris-HCl, 25 mM NaCl,(pH 7.5).Included with 20 µL and 60 µL pack sizes:200 µl 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)Molecular weight~55,000 daltonsFormulationThe enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl pH 7.5.Suggested usage1. Add up to 1 nmole of labeled oligosaccharide to a tube.2. Add de-ionized water to a total of 15 µl.3. Add 4 µl of 5x Reaction Buffer 5.0.4. Add 1 µl of Alpha-(1-6)-Fucosidase.5. Incubate for 3 hours at 37˚C.Specificityα(1-6) linked core fucose when covalently attached to a reporter molecule at the reducing terminal GlcNAc. The one exception is that a terminal unbranched α(1-3) ) or α(1-4) fucose is cleaved in the absence of any reporter molecule. Reporter molecules known to support cleavage are amino-napthalene disulfonic and tri- sulfonic acids and 2-aminobenzoic acid(2AA). However, 2-aminobenzamide(2-AB)will not support cleavage. Shorter oligosaccharides such as trimannosylchitobiose are more completely digested than longer derivatives which may require longer incubation times.Specific Activity AssayOne unit of Fucosidase activity is defined as the amount of enzyme required to cleave 1 µmole of fucose from 4-methylumbelliferyl-alpha-L-fucoside in 1 minute at 37˚C and pH 5.0.PurityEach lot of α(1-6) Fucosidase is tested for contaminating protease as follows: 10µg of denatured BSA is incubated for 24 hours with 2 µL of enzyme. Analysis of the BSA band after SDS-PAGE should show no evidence of degradation.The production host strain has been extensively tested and does not produce any detectable glycosidases.Stability Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity... Read More | Inquire | Purity>95% SDS-PAGE.FunctionB Cell Activating Factor Receptor (BAFF-R), also named tumor necrosis factor receptor superfamily member 13C, is a member of the TNFR superfamily. It is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ Purity>95% SDS-PAGE.FunctionB Cell Activating Factor Receptor (BAFF-R), also named tumor necrosis factor receptor superfamily member 13C, is a member of the TNFR superfamily. It is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFF receptor is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain and binds with high specificity to BAFF (TNFSF13B). It enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF receptor/BAFF signaling plays a critical role in B cell survival and maturation... Read More | Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport | Recombinant Human Serum Albumin (rHSA) is an active compound and possesses an identical conformation to plasma derived HSA. Recombinant Human Serum Albumin (rHSA) has no difference between rHSA and plasma derived HSA. Recombinant Human Serum Albumin (rHSAAppearance:SolidBiological Activity:Recombinant Human Serum Albumin (rHSA) is an active compound and possesses an identical conformation to plasma derived HSA. Recombinant Human Serum Albumin (rHSA) has no difference between rHSA and plasma derived HSA. Recombinant Human Serum Albumin (rHSAAppearance:SolidBiological Activity:Recombinant Human Serum Albumin (rHSA) is an active compound and possesses an identical conformation to plasma derived HSA. Recombinant Human Serum Albumin (rHSA) has no difference between rHSA and plasma derived HSA. Recombinant Human Serum Albumin (rHSA... Read More |