| Description | Anti fluorescence quencher is a reagent that can slow down fluorescence quenching. It can be used in most fluorescent dyes with simple operation. This product is recommended to be used in fixed permeabilized cells or tissues. After observation, nail polish or sealant can be used to seal the edge of Anti fluorescence quencher is a reagent that can slow down fluorescence quenching. It can be used in most fluorescent dyes with simple operation. This product is recommended to be used in fixed permeabilized cells or tissues. After observation, nail polish or sealant can be used to seal the edge of the coverslip; DAPI containing antifluorescent quencher can directly stain nuclei. Presentation：Before using the product, return it to room temperature, slightly shake and mix to avoid bubbles.1.Cell samples : ( 1 ) After dyeing, absorb the liquid. ( 2 ) Add 10-20 µL anti-quenching agent to each glass slide, cover the cover glass with cells, and let the cells contact the anti-quenching agent. When the cover glass is covered, the excess can be squeezed out. The anti-quenching agent, try not to have bubbles. ( 3 ) Fluorescence microscope observation. ( 4 ) ( optional ) : After the observation, nail polish or sealant can be used to seal the edge of the cover glass. 2. Tissue sections : ( 1 ) After the staining is completed, the staining solution is sucked off. ( 2 ) Drop 10-20 µL of anti-quenching agent on the tissue section, cover the cover glass, let the section contact the anti-quenching agent, and squeeze out the excess anti-quenching agent when cover the cover glass to avoid bubbles as much as possible. ( 3 ) Observation of tissue sections by fluorescence microscope. ( 4 ) ( optional ) : After the observation, nail polish or sealant can be used to seal the edge of the cover glass. 3.Other samples : Other samples can be operated with reference to the above samples. Matters needing attention：1. the anti fluorescence quencher has poor anti quenching effect on staining live cell membrane dyes and mitochondrial dyes. It is recommended that the anti fluorescence quencher be used to fix permeabilized cells or tissues. 2. when the anti fluorescence quencher is matched with our YF 488 dye, there may be background interference. This situation may be caused by adding too much of this product. The amount of this product should be minimized. 3. the anti fluorescence quencher may not be suitable for some dyes. Pre experiment is recommended to test the matching before the experiment. 4. for your safety and health, please wear experimental clothes and disposable gloves.Scope of application：Fluorescent Mounting Media... Read More | Es Taq DNA Polymerase is an optimized mixed enzyme of Taq and Pfu DNA Polymerase, with 5 '→ 3' DNA polymerase activity, 5 '→ 3' exonuclease activity, and 3 '→ 5' exonuclease activity. Compared with Taq DNA Polymerase, Es Taq DNA Polymerase has excellent performance of high Es Taq DNA Polymerase is an optimized mixed enzyme of Taq and Pfu DNA Polymerase, with 5 '→ 3' DNA polymerase activity, 5 '→ 3' exonuclease activity, and 3 '→ 5' exonuclease activity. Compared with Taq DNA Polymerase, Es Taq DNA Polymerase has excellent performance of high amplification efficiency and low mismatch rate, and can efficiently amplify DNA fragments. Most of the PCR products amplified with this product contain an "A" base at the 3 'end, which can be directly used for T/A cloning. This product is suitable for conventional PCR reactions and gene cloning reactions that require high fidelity. E665597Component500 UStorageE665597AEs Taq DNA Polymerase, 5 U/µL 100 µL -20℃. Avoid freeze/thaw cycle.E665597B10×PCR Buffer 1.8 mL -20℃. Avoid freeze/thaw cycle.Activity definition:Using activated salmon sperm DNA as a template/primer, the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into acidic insoluble substances is defined as 1 active unit (U) at 74 ℃ for 30 minutes.Quality control:After multiple column purifications, SDS-PAGE detected a purity of over 99%; No exogenous nuclease activity detected; PCR method for detecting residual DNA without host; Can effectively amplify single copy genes in the human genome; Store at room temperature for one month without significant changes in activity.1. PCR reaction system Reagent 50 µlReaction system Final concentration 10×PCR Buffer 5 µL 1× dNTP Mix，10 mM each 1 µL 200 µM each Forward Primer，10 µM 2 µL 0.4 µM Reverse Primer，10 µM 2 µl 0.4 µM Template DNA <0.5 µg <0.5 µg/50 µl Es Taq DNA Polymerase，5 U/µl 0.25-0.5 µl 1.25-2.5U/50 µl ddH2O up to 50 µL /Attention: The primer concentration should be between 0.1 and 1.0 as the final concentration µ M serves as a reference for setting the range. In the case of low amplification efficiency, the concentration of primers can be increased; When non-specific reactions occur, the primer concentration can be reduced to optimize the reaction system. 2. PCR reaction conditions Step Temperature Time / Pre denaturation 94℃ 2 min / Denaturation 94℃ 30 s 25-35 cycles Anneal 55-65℃ 30 s 25-35 cycles Extend 72℃ 30 s 25-35 cycles Finally extended 72℃ 2 min / Attention:1) In general experiments, if the annealing temperature is 5 ℃ lower than the melting temperature Tm of the amplification primer, and the ideal amplification efficiency cannot be achieved, the annealing temperature should be appropriately reduced; When non-specific reactions occur, increase the annealing temperature to optimize the reaction conditions.2) The extension time should be set according to the size of the amplified fragment. The amplification efficiency of Es Taq DNA Polymerase in this product is 2 kb/min.3) The number of cycles can be set based on the downstream application of the amplification product. If the number of cycles is too small, the amplification amount is insufficient; If there are too many cycles, the probability of mismatches will increase, and non-specific backgrounds will be severe. So, while ensuring product yield, the number of cycles should be minimized as much as possible... Read More | Heme Oxygenase-1-IN-1 (Compound 2) is a heme oxygenase 1 ( HO-1 ) inhibitor with an IC 50 of 0.25 µMIC50& Target:IC 50 : 0.25 µM (HO-1) | Inquire | MAP kinase substrate (MBP) is a peptide substrate for ERK 1 and ERK 2 MAP kinases. Sequence contains amino acids 95-98 of myelin basic protein (MBP) including Thr 97 within the Pro-X-Ser/Thr-Pro MAP kinase substrate consensus sequence phosphorylation site.MAP kinase substrate (MBP)is a peptide MAP kinase substrate (MBP) is a peptide substrate for ERK 1 and ERK 2 MAP kinases. Sequence contains amino acids 95-98 of myelin basic protein (MBP) including Thr 97 within the Pro-X-Ser/Thr-Pro MAP kinase substrate consensus sequence phosphorylation site.MAP kinase substrate (MBP)is a peptide substrate for ERK 1 and ERK 2 MAP kinases... Read More |