| Description | Anti fluorescence quencher is a reagent that can slow down fluorescence quenching. It can be used in most fluorescent dyes with simple operation. This product is recommended to be used in fixed permeabilized cells or tissues. After observation, nail polish or sealant can be used to seal the edge of Anti fluorescence quencher is a reagent that can slow down fluorescence quenching. It can be used in most fluorescent dyes with simple operation. This product is recommended to be used in fixed permeabilized cells or tissues. After observation, nail polish or sealant can be used to seal the edge of the coverslip; DAPI containing antifluorescent quencher can directly stain nuclei. Presentation：Before using the product, return it to room temperature, slightly shake and mix to avoid bubbles.1.Cell samples : ( 1 ) After dyeing, absorb the liquid. ( 2 ) Add 10-20 µL anti-quenching agent to each glass slide, cover the cover glass with cells, and let the cells contact the anti-quenching agent. When the cover glass is covered, the excess can be squeezed out. The anti-quenching agent, try not to have bubbles. ( 3 ) Fluorescence microscope observation. ( 4 ) ( optional ) : After the observation, nail polish or sealant can be used to seal the edge of the cover glass. 2. Tissue sections : ( 1 ) After the staining is completed, the staining solution is sucked off. ( 2 ) Drop 10-20 µL of anti-quenching agent on the tissue section, cover the cover glass, let the section contact the anti-quenching agent, and squeeze out the excess anti-quenching agent when cover the cover glass to avoid bubbles as much as possible. ( 3 ) Observation of tissue sections by fluorescence microscope. ( 4 ) ( optional ) : After the observation, nail polish or sealant can be used to seal the edge of the cover glass. 3.Other samples : Other samples can be operated with reference to the above samples. Matters needing attention：1. the anti fluorescence quencher has poor anti quenching effect on staining live cell membrane dyes and mitochondrial dyes. It is recommended that the anti fluorescence quencher be used to fix permeabilized cells or tissues. 2. when the anti fluorescence quencher is matched with our YF 488 dye, there may be background interference. This situation may be caused by adding too much of this product. The amount of this product should be minimized. 3. the anti fluorescence quencher may not be suitable for some dyes. Pre experiment is recommended to test the matching before the experiment. 4. for your safety and health, please wear experimental clothes and disposable gloves.Scope of application：Fluorescent Mounting Media... Read More | 6-Bromo-2-naphthyl β-D-glucuronide is a histochemical substrate for β-D-glucuronidase | Biochemical Test:SDS-PAGE (purity > 80%); Western blot with patient sample.Calculated Isoelectric Point:pH 8.38 | Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description: DCX (doublecortin, N-GST chimera)contains 2 doublecortin domains and belongs to the doublecortin family. It is highly expressed in neuronal cells of fetal brain, but not expressed in other fetal tissues. In the Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description: DCX (doublecortin, N-GST chimera)contains 2 doublecortin domains and belongs to the doublecortin family. It is highly expressed in neuronal cells of fetal brain, but not expressed in other fetal tissues. In the adult, it is highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. DCX is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. DCX may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. It may be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Defects in DCX are the cause of lissencephaly X-linked type 1 and subcortical band heterotopia X-linked... Read More | Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleavesRibonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies. ApplicationRibonuclease T1 has extensive applications in molecular cloning and DNA sequencing. Because of its specificity it has been a commonly used cleavage enzyme for the determination of structure, nearest neighbor frequencies, and RNA sequencing. The enzyme has further application in the preparation of nucleoside 2',3'-cyclic phosphates, the synthesis of oligonucleotides, and the removal of RNA from DNA preparations. The enzyme is also used as a non-mammalian source of RNase in various applications... Read More |