| Description | Cardiolipin (CL) is a complex lipid with a dimeric structure. It has four acyl chains and two phosphatidyl moieties. Its phosphatidyl moieties are linked to the glycerol backbone. Around 15-20 percent of the inner mitochondrial membrane accounts for cardiolipin.Cardiolipin is a unique phospholipid Cardiolipin (CL) is a complex lipid with a dimeric structure. It has four acyl chains and two phosphatidyl moieties. Its phosphatidyl moieties are linked to the glycerol backbone. Around 15-20 percent of the inner mitochondrial membrane accounts for cardiolipin.Cardiolipin is a unique phospholipid found exclusively within the mitochondrial inner membrane. This specific localization allows Cardiolipin to exert crucial influence on mitochondrial structure and function. Deviations in Cardiolipin content, structure, or localization can lead to impaired mitochondrial activity, contributing to various diseases like cancer, neurological disorders, cardiovascular conditions, and metabolic diseases. As Cardiolipin plays a crucial role in mitochondrial function, it holds immense potential for biomarker development and finds application in cardiovascular, neuroscience, cancer, diabetes, and metabolomics research.Application：Cardiolipin solution from bovine heart has been used:for coating Corning 96-well enzyme immunoassay/radioimmunoassay (EIA/RIA) plate to perform enzyme-linked immunosorbent assay (ELISA)-based cardiolipin binding assayin lipid analysis along with phosphatidylglycerol (PG) to comigrate the radiolabeled lipids to identify PG and cardiolipin (CL)in coating ELISA plates for antiphospholipid antibodies (aPL) assaysCharacteristics and advantages：High-quality molecule suitable for mulitple research applicationsCommonly employed in Metabolomics and Biochemical studies... Read More | Amine-Reactive probe which passively diffuse into cells and it is nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the highly fluorescent, amine-reactive fluorophore. Upon reaction with amine-containing residues of intracellular proteins, these probes form dye Amine-Reactive probe which passively diffuse into cells and it is nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the highly fluorescent, amine-reactive fluorophore. Upon reaction with amine-containing residues of intracellular proteins, these probes form dye protein adducts that are well retained in cells as they move and divide during embryonic development.A Non-fluorescent cell permeant amine-reactive probe for long term tracing of cell... Read More | Extinction CoeffA280 nm = 1.0 at 1.0 mg/mlGeneral DescriptionProduct is whole polyclonal antiserum from goats immunized with highly purified mouse complement protein. This product is not a purified IgG fraction. Goats are maintained in FDA certified facilities.Physical Characteristics & Extinction CoeffA280 nm = 1.0 at 1.0 mg/mlGeneral DescriptionProduct is whole polyclonal antiserum from goats immunized with highly purified mouse complement protein. This product is not a purified IgG fraction. Goats are maintained in FDA certified facilities.Physical Characteristics & StructureAntibodies present in the antisera are primarily IgG.ApplicationsImmunodiffusion: Effective against NMS and plasma at 1/32 dilution Suggested starting dilutions:Western Blot: 1/500 to 1/1000. Most effective against non-reduced antigen.ELISA: 1/500 to 1/2000... Read More | Purity>95% (SDS-PAGE) Endotoxin level<1.0 EU/µgFunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells | Product Characteristics UNI-StabilPLUS is a universal stabilizer for the dilution and stabilization of both Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP) labeled proteins and antibodies, in order to maintain the molecular conformation and prevent loss of activity over time. This enablesProduct Characteristics UNI-StabilPLUS is a universal stabilizer for the dilution and stabilization of both Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP) labeled proteins and antibodies, in order to maintain the molecular conformation and prevent loss of activity over time. This enables the making of pre-diluted, ready-to-use conjugates, minimizing assay errors in dilution. Superior stabilization of HRP and AP conjugated antibodies in low as well as high protein dilutions is seen, when using UNI-StabilPLUS. When tested with AP conjugated antibody stability is seen as follows: • at least 3 years at 2-8 °C • at least 2 years at room temperature • at least 4 weeks at 37 °C When tested with HRP conjugated antibody stability is seen as follows: • at least 2 years at 2-8 °C • at least 1 years at room temperature • at least 2 weeks at 37 °CUNI-StabilPLUS is recommended for the dilution of antibodies directed against rabbit immunoglobulins unlike HRP-StabilPLUS (cat. no. H494387) and Antibody Enhancer (cat. no. A494276).Composition & Properties UNI-StabilPLUS is a ready-to use buffer that appears as an opaque solution. The product is based on a mild acid Tris buffer containing proprietary stabilizing components. UNI-StabilPLUS contains neither BSA, nor other material from bovine serum, no azide, mercury or other toxic components.Working Procedure 1.Make a series of dilutions of the HRP- or AP conjugated protein in UNI-StabilPLUS in order to determine the optimal dilution. 2.Run the assay as usual or store the diluted conjugated protein preferably at 2-8 °C.Tips & Tricks • Avoid using phosphate buffers for AP-conjugated antibody assays. We recommend the use of Tris/HCl, Tween as the washing buffer, instead of a PBS buffer which will reduce signal significantly. • For extended stability of HRP conjugated antibodies, HRP-StabilPLUS (cat. no. H494387) is recommended. Handling & Storage • Store solution at 2-8 °C... Read More |