| Description | Extinction CoeffA280 nm = 1.35 at 1.0 mg/mLSpecificityMonospecific for human C2 in human plasma or serumGeneral DescriptionProduct is made from polyclonal antiserum from goats immunized with highly purified human complement protein. Product is the purified IgG fraction. Goats are maintained in FDA Extinction CoeffA280 nm = 1.35 at 1.0 mg/mLSpecificityMonospecific for human C2 in human plasma or serumGeneral DescriptionProduct is made from polyclonal antiserum from goats immunized with highly purified human complement protein. Product is the purified IgG fraction. Goats are maintained in FDA certified facilities.Physical Characteristics & StructureAntibodies present are primarily IgG.ApplicationsWestern Blots: Effective at dilutions 1/4,000 to 1/8,000 depending on conditions.Note: Most effective against non-reduced antigen.ELISA: Effective at dilutions 1/8,000 to 1/16,000 depending on conditions.Radial immunodiffusion: 1/44 dilution (Vol/Vol) of anti-C2 IgG fraction in agarose gel results, after staining, in clear precipitin rings with neat, 1:2 and 1:4 dilutions of NHS (3 mm wells, 20 µl sample).versus activated ½ diluted NHS assayed by immunodiffusion and staining of the precipitin band.Immunodiffusion: Effective at 1/8 dilution against NHS or plasma... Read More | Inquire | The Leuconostoc GPDH exhibits dual coenzyme specificity, namely NAD and NADP (Olive and Levy, Biochem., 6, 730 730, 1967). When assayed under conditions that are optimal for the particular coenzyme, the ratio of observed catalytic activity is NAD/NADP = 1.8 | Purity>97% by SDS-PAGE and HPLC analyses.FunctionPlays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells Purity>97% by SDS-PAGE and HPLC analyses.FunctionPlays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase... Read More | Tyrosine decarboxylase catalyzes the removal of the carboxyl group from tyrosine to produce tyramine and carbon dioxide. Pyridoxal 5'-phosphate is a necessary cofactor. By using the apoenzyme prepared from cells grown on a vitamin B6 deficient medium pyridoxal phosphate may be determined. The Tyrosine decarboxylase catalyzes the removal of the carboxyl group from tyrosine to produce tyramine and carbon dioxide. Pyridoxal 5'-phosphate is a necessary cofactor. By using the apoenzyme prepared from cells grown on a vitamin B6 deficient medium pyridoxal phosphate may be determined. The HOLOenzyme may be used to determine tyrosine, phenylalanine and dihydroxyphenylalanine either manometrically or colorimetrically.L-Tyrosine decarboxylase apoenzyme from Streptococcus faecalis has been used in a study to purify and characterize tyrosine decarboxylase and aromatic-L-amino-acid decarboxylase.L-Tyrosine decarboxylase apoenzyme from Streptococcus faecalis has also been used in a study to investigate the stereospecificity of sodium borohydride reduction of tyrosine decarboxylase.One Unit yields 1µmole of CO2 per minute from L-tyrosine at 37°C, pH 5.5. The APOenzyme activity is measured in the presence of excess pyridoxal phosphate... Read More |