| Description | Product Descriptionalpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, alpha mannosidase, exo-alpha-mannosidaseAlpha Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.This enzyme is often used in conjunction with Core Alpha-(1-6) Mannosidase (E-AM02) if aProduct Descriptionalpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, alpha mannosidase, exo-alpha-mannosidaseAlpha Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.This enzyme is often used in conjunction with Core Alpha-(1-6) Mannosidase (E-AM02) if a noncleavable core α(1-6) mannose is present on the substrate.SpecifictyAll Alpha-(1-2,3,6)-linked mannosePurityEach lot of α(1-2,3,6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 µU/mL (IUB).For the protease assay, 10 µg of denatured BSA is incubated for 24 hours with 2 µl of enzyme. Analysis of the BSA band after SDS-PAGE should show no evidence of degradation.Molecular weighttwo polypeptides of 64,000 and 44,000 daltonsSpecific Activity AssayOne unit of Alpha Mannosidase is defined as the amount of enzyme required to hydrolyze 1 µmole of p-nitrophenyl-alpha-p-mannoside to p-nitrophenol in 1 minute at pH 5.0 and 37˚C.ContentsAlpha-(1-2,3,6) Mannosidase in 150 mM sodium phosphate, 0.1 mM ZnCl2 pH 7.5. (pH 7.5).Included with 20 µL and 60 µL pack sizes:200 µl 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0).Suggested usage1. Add up to 1 nmol of oligosaccharide.2. Add deionized water to 15 µl.3. Add 4 µl 5x Reaction Buffer 5.0.4. Add 1 µL of enzyme5. Incubate ten minutes at 37˚C... Read More | Product Application：KNK437 has been used: as a heat shock factor 1 (HSF1) inhibitor to study its effects on the inhibition of viability and apoptosis activation in chemoresistant mice cells as an HSF1 inhibitor to study its effects on viability and apoptosis of colorectal cancer cells as a Product Application：KNK437 has been used: as a heat shock factor 1 (HSF1) inhibitor to study its effects on the inhibition of viability and apoptosis activation in chemoresistant mice cells as an HSF1 inhibitor to study its effects on viability and apoptosis of colorectal cancer cells as a heat shock protein 70 (HSP70) inhibitor to study its effects on glutamine-induced HSP70 and inflammatory mediator release... Read More | Inquire | Inquire | Inquire |