| Description | Product content F665904Component500 U2500 UStorageF665904AFastStar DNA Polymerase, 5 U/µL 100 µL5×100 µL-20℃. Avoid freeze/thaw cycle.F665904B10×PCR Buffer 1.8 mL5×1.8 mL-20℃. Avoid freeze/thaw cycle.Notes: 10×PCR Buffer contains 15mM Mg2+. Product Product content F665904Component500 U2500 UStorageF665904AFastStar DNA Polymerase, 5 U/µL 100 µL5×100 µL-20℃. Avoid freeze/thaw cycle.F665904B10×PCR Buffer 1.8 mL5×1.8 mL-20℃. Avoid freeze/thaw cycle.Notes: 10×PCR Buffer contains 15mM Mg2+. Product IntroductionFastStar DNA Polymerase is a mixture of anti-Taq enzyme monoclonal antibody and CW0680 Taq DNA Polymerase for Hot Start PCR.When using Taq enzyme antibody for PCR amplification, Taq enzyme antibody binds to Taq enzyme to inhibit the DNA polymerase activity prior to high temperature denaturation, and effectively inhibits non-specific annealing of primers and non-specific amplification caused by primer dimerization at low temperatures. It can effectively inhibit the non-specific annealing of primers and non-specific amplification caused by primer dimer under low temperature conditions.Taqase antibody denatures the DNA in the initial DNA denaturation step of the PCR reaction, and DNA polymerase activity is restored to achieve the effect of hot start. No special inactivation of Taq enzyme antibody is required for the use of this product, and it can be used under conventional PCR reaction conditions.FastStar DNA Polymerase with 5′→3′ DNA polymerase activity and 5′→3′ exonuclease activity, no 3′→With 5′ exonuclease activity and an enzyme extension rate of 2kb/min, fragments up to 5kb in length can be amplified. The amplified PCR product has an "A" base at the 3′ end, so it can be directly used for T/A cloning. This product is characterized by fast extension speed and high amplification efficiency, and is mainly suitable for PCR amplification of DNA fragments, DNA sequence determination and other experiments.Active DefinitionUsing activated salmon sperm DNA as template/primer, the amount of enzyme required to dope 10 nmol of deoxyribonucleotide into acid-insoluble material was defined as 1 activity unit (U) at 74°C for 30 min.quality controlAfter several column purifications, its purity was greater than 99% by SDS-PAGE; no exogenous nuclease activity was detected;PCR method detects no host residual DNA; efficiently amplifies single-copy genes in the human genome.UsageThe following example shows the PCR reaction system and reaction conditions for amplifying a 1kb fragment using human genomic DNA as a template, which should be improved and optimized according to the template, primer structure and size of the target fragment in actual operation.Reagent50 µl Reaction systemfinal concentration10× PCR Buffer5 µl1×dNTP Mix，10 mM each1 µl200 µM eachForward Primer，10 µM2 µl0.4 µMReverse Primer，10 µM2 µl0.4 µMTemplate DNA<0.5 µg<0.5 µg/50 µlFastStar DNA Polymerase,5U/µl0.25-0.5 µl1.25-2.5 U/50 µlddH₂Oup to 50 µlNote: The reaction solution can be prepared at room temperature; the reagents must be placed on ice.2.PCR reaction conditionsAttention:1) In general, the annealing temperature in the experiment is 5°C lower than the melting temperature Tm of the amplification primer, and when the ideal amplification efficiency cannot be obtained, the annealing temperature is appropriately lowered.2) The extension time should be set according to the size of the amplified fragment.3) The number of cycles can be set according to the downstream application of the amplification product. If the number of cycles is too low, the amplification is insufficient; if the number of cycles is too high, the chance of mismatch will increase and the non-specific background will be serious. Therefore, the number of cycles should be minimized under the premise of ensuring the product yield... Read More | Acid phosphatase is an esterase with broad activity at an optimal pH below 7.0. There are three isozymes, EI, EII, and EIII of similar molecular weight (55 kDa± 5 kDa). Their optimum pH's are 5.5, 4.5, and 4.0 respectively. Acid phosphatase activity was observed by Teller Aladdin Library Acid phosphatase is an esterase with broad activity at an optimal pH below 7.0. There are three isozymes, EI, EII, and EIII of similar molecular weight (55 kDa± 5 kDa). Their optimum pH's are 5.5, 4.5, and 4.0 respectively. Acid phosphatase activity was observed by Teller Aladdin Library Archives in 1954 in preparations of a wheat germ lipase described by Singer JBC, 174, 11, in 1948. Equivalent commercial preparations have been distributed labeled as lipase and acid phosphatase thus generating some confusion. Subsequent work has confirmed that the non-specific esterase activity of the wheat germ preparation may be measured both as lipase (triacetin as substrate) and phosphatase. The enzyme assay is based on the work of Brandenberger and Hanson (Helv. Chim. Acta, 36, 900, 1953) and Hofstee ( Arch. Biochem. Biophys., 51, 239, 1954).Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell.Characteristics of Acid Phosphatase from Wheat Germ:Molecular weight: 55,000 ± 5,000 (Verjee 1969).Composition: Three isozymes of closely similar molecular weights have been reported by Verjee (1969): EI, EII, and EIII. See also Brouillard and Ouellet (1965).Optimal pH: EI - 5.5, EII - 4.5, and EIII - 4.0. (Verjee 1969).Specificity: The enzyme has a broad esterase activity. See Joyce and Grisolia (1960). It shows highest activity for pyrophosphate.Inhibitors: Fluoride, molybdate and orthophosphate (Verjee 1969)... Read More | Protein:BovineEnzyme:Horseradish peroxidase | Inquire | Inquire |