| Description | Product description:Hotstart Taq DNA Polymerase High concentration is a thermostable TAQ DNA polymerase modified by an aptamer group. Before high temperature heating, the aptamer group combines with Taq enzyme to inhibit the activity of polymerase, avoid non-specific amplification of primer Product description:Hotstart Taq DNA Polymerase High concentration is a thermostable TAQ DNA polymerase modified by an aptamer group. Before high temperature heating, the aptamer group combines with Taq enzyme to inhibit the activity of polymerase, avoid non-specific amplification of primer extension or the production of primer dimer, enhance the specificity, sensitivity and stability of DNA amplification, and can be widely used in conventional PCR, multiple PCR, nested PCR, etc. After heat shock at 95 ℃ for 10 min, the enzyme can recover its activity. In addition, Hotstart Taq DNA Polymerase High concentration did not detect 3 '→ 5' exonase activity, but it has 5 '→ 3' exonase activity, which can be used for fluorescent quantitative PCR detection. Hotstart Taq DNA Polymerase High concentration has no activity at room temperature, which is convenient for the normal temperature operation of PCR experiment.Product content:1. Hotstart Taq DNA Polymerase High-concentration (10 U/ µ l )2. 5 × Hotstart Taq Buffer ( Mg²⁺ Plus)3. Solution I (10 ×)Activity definition: at 74 ℃ for 30 min, the amount of enzyme required for 10 nmol dNTP to be mixed into acid insoluble sediment is defined as one activity unit.usage method:1. Setting of PCR reaction system:a. Dissolve and mix all solutions required for PCR reaction. It shall be placed on the ice bath or in the ice box. It is recommended that reaction PCR liquid be used separately to avoid repeated freezing and thawing.b. Refer to the following table to set up PCR reaction. It is recommended that the PCR reaction system be configured in an ice bath or on an ice box:※ Dosage of template DNA: To ensure the sensitivity of reaction, 25 µ L The system uses the target sequence copied from 10⁴ as the template. Please refer to the following table to calculate the template amount to be added to the PCR system.one µg Moles of DNA from various sourcesFor example, the concentration of purified human genome DNA is 1 µ g/ µ l. The number of copies of a gene in the human genome is 10, and the number of copies per unit volume is:3.0× 10⁵ mol/µg × 1 µg/µl × 10 copy/mol=3.0× 10⁶ copy/µl1× 10⁴ copy/ (3.0× 10⁶ copy/µl)= 1/300 µlThat is, the concentration of 1/300 ul is 1 ug/ µ The human genome DNA of L contains 10⁴ copies of this gene, diluted 300 times and then added 1 µ L to 25 µ L PCR system. To ensure the specificity of the reaction, the final concentration of DNA should be less than 10 ng/ul, and excessive DNA may have smear bands or even no specific bands.c. Use a pipette to gently blow and mix or slightly Vortex and centrifuge at room temperature for several seconds to make the liquid volume concentrate at the bottom of the tube.d. Place each set PCR reaction tube on the PCR instrument to start PCR reaction.2. Setting of PCR reaction parameters:This product is a aptamer modified Hotstart Taq DNA Polymerase High concentration. In order to optimize the amplification efficiency and quantitative accuracy of PCR, it is recommended that the heat shock time be 95 ℃, 10 minutes3. Product packaging:Product composition1KU5KUStorage temperatureHotstart Taq DNA Polymerase (5 U/µl )200µl1ml-20℃5×Hotstart Taq Buffer（Mg2+ Plus）4ml20ml-20℃Solution I (10×)2ml10ml-20℃... Read More | Inquire | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：CD200 R1, also known as OX-2 receptor, is a 90 kDa transmembrane protein in the immunoglobulin superfamily and is important in the regulation of myeloid cell activity. The human CD200 R1 cDNA encodes a 325 Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：CD200 R1, also known as OX-2 receptor, is a 90 kDa transmembrane protein in the immunoglobulin superfamily and is important in the regulation of myeloid cell activity. The human CD200 R1 cDNA encodes a 325 amino acid (aa) precursor that includes a 28 aa signal sequence, a 215 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain. Within the ECD, human CD200 R1 shares 56% aa sequence identity with both mouse and rat CD200 R1. Alternate splicing of the human CD200 R1 mRNA generates four isoforms, two of which are truncated in the Ig-C2 domain and are likely secreted. In human, a separate CD200 RL gene encodes a protein that shares 81% ECD aa identity with CD200 R1. In mouse, at least four genes for CD200 R1-like molecules have been described. CD200 R1 expression is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, while its ligand, CD200, is widely distributed. Disruption of this receptor-ligand system by knockout of the CD200 gene in mice leads to increased macrophage number and activation and predisposition to autoimmune disorders. Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains. The capacity of CD200 R1-like molecules to interact with CD200 is controversial. CD200 R1 propagates inhibitory signals despite lacking a cytoplasmic ITIM (immunoreceptor tyrosine-based inhibitory motif). CD200 R1-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12. CD200R1 signaling inhibits the expression of proinflammatory molecules including TNFs, IFNs, and inducible nitric oxide synthase in response to selected stimuli, which implicate that CD200/CD200R1 inhibitory signaling pathway plays a prominent role in limiting inflammation in a wide range of inflammatory diseases. Furthermore, the CD200/CD200R inhibitory signaling constitutes one of the most suitable endogenous immunoregulatory molecule candidate to restore the immune suppressive status of the CNS altered in chronic neuroinflammatory situations... Read More | As the most abundant protein in human plasma, human serum albumin (HSA) is the transporter of hormones, lipids and other substances. Its main physiological function is to regulate plasma pH and maintain plasma osmotic pressure.Osrhsa (recombinant human serum albumin from Oryza sativa) is a As the most abundant protein in human plasma, human serum albumin (HSA) is the transporter of hormones, lipids and other substances. Its main physiological function is to regulate plasma pH and maintain plasma osmotic pressure.Osrhsa (recombinant human serum albumin from Oryza sativa) is a recombinant human serum albumin developed by using rice endosperm cell expression platform (oryzhiexp) and purification platform (oryzpur). It does not contain animal derived ingredients and can eliminate the risk of blood derived virus infection. Compared with fetal bovine serum (FBS), plasma derived albumin (pHSA) and bovine serum albumin (BSA), osrhsa has higher purity and better batch stability. It can be used in various research fields, including biopharmaceutical, cell therapy and cell culture of gene therapy. It can replace serum and promote cell growth. At the same time, osrhsa is also widely used in biomedical production as drug carrier, vaccine protector, cell cryoprotectant and medical device embedding agent.ApplicationBiopharmaceuticals, human vaccines, cell culture, cell storage, chemical drug molecular carriers, medical devices, in vitro diagnosis, etc.Comparison of physical and chemical properties between OsrHSA and natural human white pHSAphysicochemical propertiespHSAOsrHSAamino acid sequenceagreementN-terminal amino acidsDAHKSEVDAHKSEVC-terminal amino acidsKLVAASQAALGLKLVAASQAALGLGlycoside modificationnothingmolecular weight (MALDl)66.554 (kDa)66.550 ( a)Isoelectric point (pl)4.84.8Drug binding activityclosethermal stabilitymp 65℃mp 65℃esterase activityidenticalcrystal structureidenticalRestrictions on use:The above products are only suitable for scientific research, laboratory and production use, and cannot be directly used in human body... Read More | Purity>95% SDS-PAGE.Additional sequence informationFull length mature chain without signal peptide.FunctionLineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be Purity>95% SDS-PAGE.Additional sequence informationFull length mature chain without signal peptide.FunctionLineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets... Read More |