| Description | Absorbance: A412 nm = 0.87 at 1/50 dilution in deionized waterGeneral DescriptionSheep red blood cells which have been washed free of sheep complement proteins, but were not coated with hemolysin antibodies. These cells have traditionally been used as negative controls for assays of the human Absorbance: A412 nm = 0.87 at 1/50 dilution in deionized waterGeneral DescriptionSheep red blood cells which have been washed free of sheep complement proteins, but were not coated with hemolysin antibodies. These cells have traditionally been used as negative controls for assays of the human classical and alternative complement pathways in serum samples (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)). Most human serum samples have small amounts of natural antibodies (usually IgG) to sheep antigens and at high concentrations of serum will agglutinate Es and activate the classical pathway leading to lysis. This rarely occurs at dilutions used in the CH50 titration assay where serum is diluted more than 1/100. Es are supplied at assay-ready concentrations in the traditional buffer used in CH50 assays (GVB⁺⁺). They can usually be used for 2 weeks after preparation. They are shipped cold, but are not harmed by extended periods at room temperature (note that they circulate 60-90 days a 37℃ in vivo). They should be washed once before each use (5 min at 500 to 1000 x g at 4℃) and resuspended in GVB++ to reduce background. This procedure may also be used to concentrate the cells.Physical CharacteristicsEs are natural uncoated erythrocytes that do not activate complement under typical assays conditions. They do not have IgG or IgM on their surface, however, there are natural antibodies in most animal serum. In the most sensitive assays it has been estimated that as few as 10 IgM/EA can cause lysis while this requires approximately 1000 IgG/EA (Ross, G.D. (1986)). This is primarily because in IgM there are five closely spaced Fc domains in fixed positions on each molecule. The spacing of IgG molecules is more random and it is relatively rare to find two or more IgG in the correct positions on the surface of a cell.ApplicationsEs cells are primarily used as controls for CH50 assays and for specialized tests requiring uncoated erythrocytes. Natural antibodies present in human blood to animal antigens may cause agglutination of the cells and lysis if the serum is used at high concentrations, however, this does not interfere with the CH50 titers because EA used in these assays already carry high levels of IgM antibodies and serum is diluted 100-fold or more. IgG antibodies are 100-fold less active than IgM and most of the cross-reactive antibodies in human blood are IgG.RegulationSheep erythrocytes (Es) are used for human complement assays partly for convenience, but also because they lack membrane-bound regulators of human complement. No significant level of functional DAF, CD59 or CR1 exists on Es for human complement. Thus, Es are useful for their lack of membrane regulatory activities... Read More | Nucleoprotein (396-404) is the 396 to 404 fragment of lymphocytic choriomeningitis virus (LCMV). Nucleoprotein (396-404) is the H-2D(b)-restricted immunodominant epitope and can be used as a molecular model of viral antigen.Form:Solid | Inquire | Purity>95% by SDS-PAGE and HPLC analyses.FunctionLigand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6Purity>95% by SDS-PAGE and HPLC analyses.FunctionLigand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation... Read More | As the most abundant protein in human plasma, human serum albumin (HSA) is the transporter of hormones, lipids and other substances. Its main physiological function is to regulate plasma pH and maintain plasma osmotic pressure.Osrhsa (recombinant human serum albumin from Oryza sativa) is a As the most abundant protein in human plasma, human serum albumin (HSA) is the transporter of hormones, lipids and other substances. Its main physiological function is to regulate plasma pH and maintain plasma osmotic pressure.Osrhsa (recombinant human serum albumin from Oryza sativa) is a recombinant human serum albumin developed by using rice endosperm cell expression platform (oryzhiexp) and purification platform (oryzpur). It does not contain animal derived ingredients and can eliminate the risk of blood derived virus infection. Compared with fetal bovine serum (FBS), plasma derived albumin (pHSA) and bovine serum albumin (BSA), osrhsa has higher purity and better batch stability. It can be used in various research fields, including biopharmaceutical, cell therapy and cell culture of gene therapy. It can replace serum and promote cell growth. At the same time, osrhsa is also widely used in biomedical production as drug carrier, vaccine protector, cell cryoprotectant and medical device embedding agent.ApplicationBiopharmaceuticals, human vaccines, cell culture, cell storage, chemical drug molecular carriers, medical devices, in vitro diagnosis, etc.Comparison of physical and chemical properties between OsrHSA and natural human white pHSAphysicochemical propertiespHSAOsrHSAamino acid sequenceagreementN-terminal amino acidsDAHKSEVDAHKSEVC-terminal amino acidsKLVAASQAALGLKLVAASQAALGLGlycoside modificationnothingmolecular weight (MALDl)66.554 (kDa)66.550 ( a)Isoelectric point (pl)4.84.8Drug binding activityclosethermal stabilitymp 65℃mp 65℃esterase activityidenticalcrystal structureidenticalRestrictions on use:The above products are only suitable for scientific research, laboratory and production use, and cannot be directly used in human body... Read More |