| Description | Products contentS665683Component1mL5mLStorageS665683A2×Super Kfx MasterMix1 mL5×1 mL -20℃. Avoid freeze/thaw cycle.S665683BddH2O1 mL5×1 mL -20℃. Avoid freeze/thaw cycle.Products IntroductionThis product is a premixed system consisting of Super Kfx DNA Polymerase, Mg2+, Products contentS665683Component1mL5mLStorageS665683A2×Super Kfx MasterMix1 mL5×1 mL -20℃. Avoid freeze/thaw cycle.S665683BddH2O1 mL5×1 mL -20℃. Avoid freeze/thaw cycle.Products IntroductionThis product is a premixed system consisting of Super Kfx DNA Polymerase, Mg2+, dNTPs, PCR stabilizers and enhancers at a concentration of 2×. Super Kfx DNA Polymerase is a fast, high-fidelity DNA polymerase with high amplification efficiency, which possesses 5′-3′DNA polymerase activity and 3′-5′ exonuclease activity. With 5′-3′ DNA polymerase activity and 3′-5′ exonuclease activity, the enzyme has the advantages of strong amplification ability, high fidelity and high specificity, etc. 2×Mix has added unique amplification enhancement factors and extension factors, and the unique formula makes the whole reaction system very stable and easy to operate, which is suitable for the amplification of various fragments and templates. The product is suitable for gene cloning, second generation library amplification, targeted gene mutation, SNP amplification experiments. quality controlNo exogenous nuclease activity, can effectively amplify various templates; stored at 2-8℃ for one month, no obvious activity change.UsageThe following examples are conventional PCR reaction systems and conditions, which should be improved and optimized according to the template, primer structure and target fragment size.1. PCR reaction system All operations should be carried out on ice, please mix the components thoroughly after thawing, after use, please promptly return to -20 ℃ for storage. 2. PCR reaction system take note of1）Priority is given to three-step amplification; if the three-step method fails to amplify the target product or if the primer Tm value is greater than 68°C, try the two-step method.2）Denaturation: pre-denaturation of simple templates 98°C, 30s-1min, for complex templates, the pre-denaturation time can be extended to 3min. 3）Annealing: In general, the annealing temperature is 3-5℃ lower than the Tm value of the primers. If the desired amplification efficiency cannot be obtained, the annealing temperature should be changed in a gradient to optimize the results; if a non-specific reaction occurs, the annealing temperature should be increased appropriately.4）Extension: The extension time should be set according to the length of the amplified fragments and the complexity of the template, the amplification efficiency of this product is 4-6kb/min, for long fragments and templates with high complexity it is recommended that 2-4kb/min. 5）Cycling times: the number of cycles can be set according to the downstream application of the amplified product. If the number of cycles is too small, the amplification will be insufficient, and if the number of cycles is too large, the chance of mismatch will be increased, so the number of cycles should be minimized under the premise of guaranteeing the yield of the product... Read More | Inquire | Inquire | Purity>97% SDS-PAGE.FunctionReceptor for interleukin-2 | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue StainingDescription:NG2, also known as CSPG4, MCSP, and AN2, is a 400-500 kDa transmembrane chondroitin sulfate proteoglycan (CSPG) with a protein core of approximately 300 kDa. The extracellular region can be proteolytically shed fromPurity:>95%, by SDS-PAGE visualized with Coomassie® Blue StainingDescription:NG2, also known as CSPG4, MCSP, and AN2, is a 400-500 kDa transmembrane chondroitin sulfate proteoglycan (CSPG) with a protein core of approximately 300 kDa. The extracellular region can be proteolytically shed from the cell surface. Mature human NG2 consists of a 2195 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain. Within aa 1583-2224, human NG2/CSPG4 shares 83% aa sequence identity with mouse and rat CSPG4. NG2 binds to the extracellular matrix proteins Laminin, Tenascin, and Collagens II, V, and VI as well as to the growth factors FGF-2 and PDGF-AA. NG2 is expressed on glial cell progenitors known as O2A cells or NG2 glia. These cells are neuronally responsive and differentiate primarily into oligodendrocytes but also into astrocytes. NG2 associates with PDGF R alpha and the AMPA R subunit GluR2. It is up-regulated on microglial cells during inflammation and contributes to the induction of inflammatory mediators. Various CSPGs in the brain inhibit neurite outgrowth through interactions with Nogo Receptor/NgR1 and NgR3. This recombinant protein product corresponds to the last 5 CSPG repeats, a region which can independently inhibit neurite outgrowth. NG2 is also expressed on vascular mural cells and capillaries. It promotes vascular endothelial cell (EC) migration and angiogenesis through interactions with Galectin-3 and Integrin alpha 3 beta 1 on EC, Plasminogen, and Angiostatin. NG2 is also expressed on a variety of tumors where it contributes to tumor cell adhesion, motility, and invasion... Read More |