| Description | Products Content:E666128Component4 KU20 KUStorageE666128AExonuclease I, 20 U/µL200 µL5×200 µL-20℃. Avoid freeze/thaw cycle.E666128B10×Exo I Reaction Buffer1 mL5×1 mL-20℃. Avoid freeze/thaw cycle.Products Introduction This product is derived from a Products Content:E666128Component4 KU20 KUStorageE666128AExonuclease I, 20 U/µL200 µL5×200 µL-20℃. Avoid freeze/thaw cycle.E666128B10×Exo I Reaction Buffer1 mL5×1 mL-20℃. Avoid freeze/thaw cycle.Products Introduction This product is derived from a recombinant E.coli strain that carries the Exo I gene, which has an exonuclease activity that hydrolyzes single-stranded DNA in the 3'-5' direction, and is capable of gradually releasing the deoxyribonucleic acid 5' monophosphate, leaving the 5' end of the di-nucleotide intact. The product is mainly used for PCR amplification. It is mainly used to degrade digested primers after PCR amplification, and is inactive on double-stranded DNA and 3' OH-terminal DNA strands enclosed by phosphoryl or acetyl groups. Active DefinitionThe amount of enzyme required to catalyze the release of 10 nmol of soluble nucleotide in 30 minutes at 37°C is defined as 1 unit of activity (U). quality controlThe purity of the enzyme is more than 95% after SDS-PAGE electrophoresis and analyzed by Caulmers Brilliant Blue staining. The addition of BSA can ensure the stability of the enzyme. UsageThe following is an example of PCR product cleanup prior to sequencing. This reaction removes single-stranded primers and degrades unpaired nucleotides. 1. Mix the PCR product with Exonuclease I according to the table below. 2. Mix and incubate at 37°C for 30 minutes.3. Inactivate by incubation at 80°C for 20 minutes... Read More | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc | Purity> 96% by SDS-PAGE and HPLC analyses.FunctionHas weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, Purity> 96% by SDS-PAGE and HPLC analyses.FunctionHas weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.Post-translationalThe N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids... Read More | IFN-αs are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-α family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166IFN-αs are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-α family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end... Read More | Purity≥95% SDS-PAGE. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.FunctionPro-inflammatory cytokine. Involved in the innate immune response to Purity≥95% SDS-PAGE. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.FunctionPro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity... Read More |