| Description | Product introduction1. Specifications: 5mM 50 µL (suitable for 4 96-well experiments), 200 µL (suitable for 16 96-well experiments)2. Parameters: MW= 412.49; Wavelength: Ex/Em= 646/697nm; Solvent: Water3. Storage: 4 ℃ protected from light for 2 yearsProduct descriptionDRAQ5 is a far-Product introduction1. Specifications: 5mM 50 µL (suitable for 4 96-well experiments), 200 µL (suitable for 16 96-well experiments)2. Parameters: MW= 412.49; Wavelength: Ex/Em= 646/697nm; Solvent: Water3. Storage: 4 ℃ protected from light for 2 yearsProduct descriptionDRAQ5 is a far-infrared fluorescent living cell DNA dye with high affinity for double-stranded DNA. It is a dye that can penetrate the cell membrane and can mark live cells or fixed/dead cells. In flow cytometry, this dye can be used to distinguish between nucleated and non-nucleated cells. Because DRAQ5 can bind to DNA in a stoichiometric ratio, it can also be used to report nuclear DNA content, and is suitable for chromosome multiple and cell cycle analysis. In fluorescence microscopy analysis, it can be used as a nuclear counterstain. DRAQ5 has many applications and is highly compatible with programs widely used in existing instrument platforms, the main application areas are HCS, cell models, GFP, flow cytometry and fluorescence microscopes.The excitation wavelength range of DRAQ5 is 488～647nm. For imaging microscopy, it is recommended to use a 633 or 647nm light source for excitation. For flow cytometry, when the dye is excited at 488nm, the 685LP dichroic mirror and 710/50 channel can be used for detection; when excited at 633nm, the 660/20 channel can be used for detection. For cell cycle/DNA analysis applications, it is recommended to use a longer wavelength filter, such as 735LP dichroic mirror and 780/60 channel to optimize the CV value of G1 and G2/M peaks. Please make sure that your instrument can detect the dye.Due to the wide emission and excitation wavelength range of DRAQ, it is not recommended to combine DRAQ5 with other far-red fluorescent dyes that can be excited at 488 or 633 nm.Instructions(1) DescriptionIn the experiment, DRAQ5 is used as the last dye to stain, because DRAQ5 does not require the remaining washing steps after staining, so DRAQ5 can be directly added to the cell-containing medium for live cell staining(2) Operation①. Prepare PBS buffer without sodium azide or a specific medium for specific cells.②. Resuspend the cells in PBS or medium, and control the cell density to ≤ 4 × 105 cells/mL. For adherent cells and some tissues, roughly estimate the number of cells.③. Add the appropriate volume of DRAQ5 staining solution of appropriate concentration according to Table 1. DRAQ5 staining solution can be directly added to the surface of tissues or adherent cells, or directly added to fresh medium④. Mix gently, and incubate at room temperature for 5-30 minutes. Incubate at 37°C and shorten the time to 1-3min. For experiments with a long time span, such as the EGFP experiment, DRAQ5 staining solution should be added to the medium during the experiment before the agonist and antagonist are added (usually 0.5 to 3 hours), and the concentration is controlled at 1 µM. Note: If the cells have been stained with other fluorescent dyes before DRAQ5 staining, please keep away from light during the above operation.⑤. The stained cells can be directly analyzed accordingly, without other operations such as washing. The following table shows the number of cells and the required volume and final concentration of DRAQ5.No. of cells:in volume:5 µM10 µM20 µM1 × 1062500 µl2.5 µl5 µl10 µl4 × 1051000 µl1 µl2 µl4 µl2 × 105500 µl0.5 µl1 µl2 µl1 × 105250 µl0.25 µl0.5 µl1 µl5 × 104125 µl0.13 µl0.25 µl0.5 µl... Read More | Extinction Coeff.A280 nm = 1.0 at 1.0 mg/mLSpecificityMonospecific for Factor B in human plasma and serumGeneral DescriptionProduct is whole polyclonal antiserum from goats immunized with highly purified human complement protein. Product is not a purified IgG fraction. Goats are maintained in FDA Extinction Coeff.A280 nm = 1.0 at 1.0 mg/mLSpecificityMonospecific for Factor B in human plasma and serumGeneral DescriptionProduct is whole polyclonal antiserum from goats immunized with highly purified human complement protein. Product is not a purified IgG fraction. Goats are maintained in FDA certified facilities.Physical Characteristics & StructureAntibodies present in the antisera are primarily IgGApplicationsWestern Blots: Effective at dilutions 1/4,000 to 1/8,000 depending on conditions.Most effective against non-reduced antigen.ELISA: Effective at dilutions 1/8,000 to 1/16,000 depending on conditions.Immunodiffusion: Effective against NHS and plasma at 1/16 dilution... Read More | Inquire | Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. Mature human CD4 consists of a 371 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain. Within the ECD, human CD4 shares approximately 52% aa sequence identity with mouse and rat CD4. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity. CD4 is reexpressed on circulating CD8+ T cells upon activation and contributes to their cytotoxic effector activity. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse. CD4 binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1... Read More | Protein:BovineEnzyme:Horseradish peroxidase |