| Description | Product introduction1. Specifications: 5mM 50 µL (suitable for 4 96-well experiments), 200 µL (suitable for 16 96-well experiments)2. Parameters: MW= 412.49; Wavelength: Ex/Em= 646/697nm; Solvent: Water3. Storage: 4 ℃ protected from light for 2 yearsProduct descriptionDRAQ5 is a far-Product introduction1. Specifications: 5mM 50 µL (suitable for 4 96-well experiments), 200 µL (suitable for 16 96-well experiments)2. Parameters: MW= 412.49; Wavelength: Ex/Em= 646/697nm; Solvent: Water3. Storage: 4 ℃ protected from light for 2 yearsProduct descriptionDRAQ5 is a far-infrared fluorescent living cell DNA dye with high affinity for double-stranded DNA. It is a dye that can penetrate the cell membrane and can mark live cells or fixed/dead cells. In flow cytometry, this dye can be used to distinguish between nucleated and non-nucleated cells. Because DRAQ5 can bind to DNA in a stoichiometric ratio, it can also be used to report nuclear DNA content, and is suitable for chromosome multiple and cell cycle analysis. In fluorescence microscopy analysis, it can be used as a nuclear counterstain. DRAQ5 has many applications and is highly compatible with programs widely used in existing instrument platforms, the main application areas are HCS, cell models, GFP, flow cytometry and fluorescence microscopes.The excitation wavelength range of DRAQ5 is 488～647nm. For imaging microscopy, it is recommended to use a 633 or 647nm light source for excitation. For flow cytometry, when the dye is excited at 488nm, the 685LP dichroic mirror and 710/50 channel can be used for detection; when excited at 633nm, the 660/20 channel can be used for detection. For cell cycle/DNA analysis applications, it is recommended to use a longer wavelength filter, such as 735LP dichroic mirror and 780/60 channel to optimize the CV value of G1 and G2/M peaks. Please make sure that your instrument can detect the dye.Due to the wide emission and excitation wavelength range of DRAQ, it is not recommended to combine DRAQ5 with other far-red fluorescent dyes that can be excited at 488 or 633 nm.Instructions(1) DescriptionIn the experiment, DRAQ5 is used as the last dye to stain, because DRAQ5 does not require the remaining washing steps after staining, so DRAQ5 can be directly added to the cell-containing medium for live cell staining(2) Operation①. Prepare PBS buffer without sodium azide or a specific medium for specific cells.②. Resuspend the cells in PBS or medium, and control the cell density to ≤ 4 × 105 cells/mL. For adherent cells and some tissues, roughly estimate the number of cells.③. Add the appropriate volume of DRAQ5 staining solution of appropriate concentration according to Table 1. DRAQ5 staining solution can be directly added to the surface of tissues or adherent cells, or directly added to fresh medium④. Mix gently, and incubate at room temperature for 5-30 minutes. Incubate at 37°C and shorten the time to 1-3min. For experiments with a long time span, such as the EGFP experiment, DRAQ5 staining solution should be added to the medium during the experiment before the agonist and antagonist are added (usually 0.5 to 3 hours), and the concentration is controlled at 1 µM. Note: If the cells have been stained with other fluorescent dyes before DRAQ5 staining, please keep away from light during the above operation.⑤. The stained cells can be directly analyzed accordingly, without other operations such as washing. The following table shows the number of cells and the required volume and final concentration of DRAQ5.No. of cells:in volume:5 µM10 µM20 µM1 × 1062500 µl2.5 µl5 µl10 µl4 × 1051000 µl1 µl2 µl4 µl2 × 105500 µl0.5 µl1 µl2 µl1 × 105250 µl0.25 µl0.5 µl1 µl5 × 104125 µl0.13 µl0.25 µl0.5 µl... Read More | description：Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast, Pichia pastoris, to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials.Bovine pancreas is a rich source of RNase A which is often found in many description：Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast, Pichia pastoris, to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials.Bovine pancreas is a rich source of RNase A which is often found in many commercial DNase preparations. Producing DNase I by recombinant means in an organism with much lower levels of endogenous RNase greatly facilitates purification of an enzyme with undetectable levels of RNase. The processes involved in the production and isolation of recombinant DNase I are completely devoid of animal based components which eliminates the possibility of introducing animal derived pathogens into bioprocessing procedures.Animal Free/AF. Recombinant Bovine pancreatic deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases & proteases. A liquid preparation in 5mM Calcium Acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol. Supplied with 10x reaction buffer.Storage Buffer : 5mM calcium acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.DNase I Reaction Buffer (10X): 500mM Tris-HCl, 10mM MgSO4, 1mM CaCl2, pH 7.8, provided.application：Recombinant DNase I is suitable for such applications as:• Removing genomic DNA from RNA preparations prior to RT-PCR• Degradation of DNA templates after transcription reactions• Removing unwanted DNA from samples prior to Northern blotting• Removing DNA during biopharma and bioprocessing procedures... Read More | EPOCROSTM is a reactive polymer with an oxazoline group on the side chain and is used as a cross-linking agent for water-based resins. Among the water-based polymers developed to address environmental issues and the increasing use of water-based products due to VOC regulations and desolventing, the EPOCROSTM is a reactive polymer with an oxazoline group on the side chain and is used as a cross-linking agent for water-based resins. Among the water-based polymers developed to address environmental issues and the increasing use of water-based products due to VOC regulations and desolventing, the EPOCROSTM WS series is a “water-soluble type” with the following structure.Features and PropertiesHigher reactivity than water-based epoxy, melamine, blocked isocyanateVOC free (EPOCROS™ WS-300 and EPOCROS™ WS-700)High crosslinking density with a small amount addedOne-pack type with long usage timeImproves water resistance, solvent resistance, heat resistance, and the strength of films, etc.Adhesion-imparting possible to PET, OPP, PVC, etc.Fast dryingLow toxicity (Ames Test: Negative, Primary Skin Irritation Test: No irritation)WS Series Product LineupApplicationsNonwoven fabric bindersPigment printingCoatings (metals, films, leather)Paint and coatings, Primers (plastics, building materials, vehicles)AdhesivesMethodASSAY for Product Code DILW:One unit equals a decrease in absorbance of 1.0 per minute at 25°C at pH 7.5 with 2,6-dichlorophenolindophenol as the chromogen.Reagents0.2 M Tris⋅HCl buffer, pH 7.50.006 M NADH. Prepare fresh daily.0.0012 M Dichlorophenolindophenol (DCPIP) Prepare fresh daily.EnzymePrepare a 10 mg/ml solution of enzyme in 0.2 M Tris⋅HCl, pH 7.5.Dilute further immediately before use to give ΔA/min of 0.15-0.20.ProcedureAdjust spectrophotometer to 600 nm and 25°C.Pipette into cuvettes as follows:Mix quickly and measure the decrease in absorbance at 600 nm for 2-3 minutes.Determine the ΔA/min. from the initial linear portion of the curve. (Use portion of curve from t=0 to t=1 minute; the rate is linear for 1/2 to 1 minute.)Calculation... Read More | Inquire | Inquire |