| Description | Product descriptionCompositionStorage Conditions50T100TSolution ARoom temperature100mL200mLDetox BRoom temperature25g50gAfter the product is received, store the ingredients at the temperature indicated above, and it will be valid for 12 months. Ethidium bromide (EB) Ames test results show that EB Product descriptionCompositionStorage Conditions50T100TSolution ARoom temperature100mL200mLDetox BRoom temperature25g50gAfter the product is received, store the ingredients at the temperature indicated above, and it will be valid for 12 months. Ethidium bromide (EB) Ames test results show that EB can easily cause mutations in organisms. Powerful EB Detoxifier is a product specially designed to remove ethidium bromide (EB) pollution. It can effectively destroy the structure of EB and remove the carcinogenicity of EB, thereby achieving the purpose of cleaning EB pollution. It is suitable for removing EB contamination of electrophoresis buffer, biochemical solution and solid surface (such as laboratory bench, centrifuge, glassware, stainless steel products, etc.). After using a powerful EB detoxifier to treat EB pollutants, then discarding it can protect the environment from EB pollutants.Product performance indexIt can destroy the structure of EB, eliminate the fluorescence of EB, and reduce its mutagenicity by more than 99.5%.Precautions1. Solution A is corrosive, and to protect your safety during the operation of EB, please wear gloves and goggles.2. There may be a small amount of irritating and harmful gas generated during the chemical reagent preparation and processing of EB, please operate in a fume hood.3. There is no method that can eliminate EB 100%, so even after handling, you should wear gloves and handle it carefully, not as 100% safe. If conditions permit, it is best to regularly test for mutagenicity to ensure the correctness of the treatment process.Operation steps: (Please read the notes before experiment)1. Treatment of various pollution solutions (100mL EB pollution solution)1) Ensure that the concentration of EB in various polluted solutions does not exceed 0.5mg/mL. If the concentration is too high, first dilute with water to meet the required concentration.2) Preparation of working solution: In a fume hood, dilute 2 mL of solution A with deionized water to a final volume of 20 mL for use. Dissolve 0.42 g of detoxifier B in water and dilute to 12 mL for use.3) Add the above 20mL solution A working solution and 12mL detoxifier B working solution to 100mL EB contaminated solution, stir and mix carefully (make sure pH≤3).4) Allow to react at room temperature for 24 hours, adjust the pH to 5-9 with sodium bicarbonate.5) Wash the reactants into the water tank with a lot of water for disposal.2. Various solid surface pollution treatment1) Preparation of working solution: In a fume hood, add 4.2g of detoxifier B to 300mL of deionized water, add 20mL of solution A after fully dissolving, stir and mix carefully (pH is about 1.8).2) After ensuring that the electrical appliances are in a power-off state, soak the freshly prepared working fluid with a paper towel, carefully wipe clean the contaminated surface, repeat 6 times, each time change to a new paper towel soaked in the working fluid, and finally soak it clean Wipe clean the working fluid with paper towels of deionized water, and collect the paper towels into a designated processing container. The pH value of the working fluid is 1.8, which is slightly corrosive. It is not suitable for wiping items with weak tolerance. You can use paper towels soaked in deionized water to wipe. Before wiping, a UV lamp can be used to help find the contaminated area. After wiping, it can help confirm that it has been wiped clean.3) Soak these contaminated paper towels in the working fluid at room temperature for at least one hour. After adjusting the pH to 5-9 with sodium bicarbonate, the liquid is flushed into the sink with plenty of water, and the paper towels are put into the garbage dump... Read More | Inquire | Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description:Cyclophilin B (SCYLP, CyPB, and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description:Cyclophilin B (SCYLP, CyPB, and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, it mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization and facilitating the activity of additional chaperones. The human CyPB precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27‑34), a PPIase domain (aa 47‑204), and a C-terminal ER retention motif (aa 213‑216). Over aa 34‑216, the human and mouse sequences are 95% aa identical... Read More | Purity>95% as determined by SDS-PAGE and Coomassie blue stainingFunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.Sequence Purity>95% as determined by SDS-PAGE and Coomassie blue stainingFunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.Sequence similaritiesBelongs to the heparin-binding growth factors family.Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1... Read More | Inquire |