| Description | Product descriptionCompositionStorage Conditions50T100TSolution ARoom temperature100mL200mLDetox BRoom temperature25g50gAfter the product is received, store the ingredients at the temperature indicated above, and it will be valid for 12 months. Ethidium bromide (EB) Ames test results show that EB Product descriptionCompositionStorage Conditions50T100TSolution ARoom temperature100mL200mLDetox BRoom temperature25g50gAfter the product is received, store the ingredients at the temperature indicated above, and it will be valid for 12 months. Ethidium bromide (EB) Ames test results show that EB can easily cause mutations in organisms. Powerful EB Detoxifier is a product specially designed to remove ethidium bromide (EB) pollution. It can effectively destroy the structure of EB and remove the carcinogenicity of EB, thereby achieving the purpose of cleaning EB pollution. It is suitable for removing EB contamination of electrophoresis buffer, biochemical solution and solid surface (such as laboratory bench, centrifuge, glassware, stainless steel products, etc.). After using a powerful EB detoxifier to treat EB pollutants, then discarding it can protect the environment from EB pollutants.Product performance indexIt can destroy the structure of EB, eliminate the fluorescence of EB, and reduce its mutagenicity by more than 99.5%.Precautions1. Solution A is corrosive, and to protect your safety during the operation of EB, please wear gloves and goggles.2. There may be a small amount of irritating and harmful gas generated during the chemical reagent preparation and processing of EB, please operate in a fume hood.3. There is no method that can eliminate EB 100%, so even after handling, you should wear gloves and handle it carefully, not as 100% safe. If conditions permit, it is best to regularly test for mutagenicity to ensure the correctness of the treatment process.Operation steps: (Please read the notes before experiment)1. Treatment of various pollution solutions (100mL EB pollution solution)1) Ensure that the concentration of EB in various polluted solutions does not exceed 0.5mg/mL. If the concentration is too high, first dilute with water to meet the required concentration.2) Preparation of working solution: In a fume hood, dilute 2 mL of solution A with deionized water to a final volume of 20 mL for use. Dissolve 0.42 g of detoxifier B in water and dilute to 12 mL for use.3) Add the above 20mL solution A working solution and 12mL detoxifier B working solution to 100mL EB contaminated solution, stir and mix carefully (make sure pH≤3).4) Allow to react at room temperature for 24 hours, adjust the pH to 5-9 with sodium bicarbonate.5) Wash the reactants into the water tank with a lot of water for disposal.2. Various solid surface pollution treatment1) Preparation of working solution: In a fume hood, add 4.2g of detoxifier B to 300mL of deionized water, add 20mL of solution A after fully dissolving, stir and mix carefully (pH is about 1.8).2) After ensuring that the electrical appliances are in a power-off state, soak the freshly prepared working fluid with a paper towel, carefully wipe clean the contaminated surface, repeat 6 times, each time change to a new paper towel soaked in the working fluid, and finally soak it clean Wipe clean the working fluid with paper towels of deionized water, and collect the paper towels into a designated processing container. The pH value of the working fluid is 1.8, which is slightly corrosive. It is not suitable for wiping items with weak tolerance. You can use paper towels soaked in deionized water to wipe. Before wiping, a UV lamp can be used to help find the contaminated area. After wiping, it can help confirm that it has been wiped clean.3) Soak these contaminated paper towels in the working fluid at room temperature for at least one hour. After adjusting the pH to 5-9 with sodium bicarbonate, the liquid is flushed into the sink with plenty of water, and the paper towels are put into the garbage dump... Read More | Inquire | Inquire | Purity> 95 % by SDS-PAGE and HPLC analyses.FunctionPromotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix Purity> 95 % by SDS-PAGE and HPLC analyses.FunctionPromotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.Banckground:Cyr61, also known as CCN1, is a 40-45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4-6). Plasmin cleavage of ECM-bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is up-regulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8-12). In contrast, whendown-regulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also up-regulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16)... Read More | Purity: >90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Function：Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence |