| Description | The 100bp DNA ladder is composed of 11 linear double stranded DNA bands, which is suitable for accurately judging the size of DNA fragments in the range of 100 bp-1500 BP in agarose gel electrophoresis, and is not recommended for acrylamide gel electrophoresis. This product is ready to use The 100bp DNA ladder is composed of 11 linear double stranded DNA bands, which is suitable for accurately judging the size of DNA fragments in the range of 100 bp-1500 BP in agarose gel electrophoresis, and is not recommended for acrylamide gel electrophoresis. This product is ready to use and contains 1 × Loading buffer can directly load samples for electrophoresis according to experimental needs, which is convenient to use and has clear electrophoresis images. Among them, the 500 BP band is shown as a bright bandRecommended electrophoretic buffer:Recommended electrophoresis buffer：TBEMatters needing attention：1.Please instantaneously centrifuge the product to the bottom of the tube before use, and then carry out subsequent experiments.2.Please replace the electrophoresis buffer in time and use the new gel to avoid affecting the electrophoresis results.3.Avoid repeated freezing and thawing.Instruction：1.Electrophoresis can be carried out with GelstainRed, and 5µL is added to the sample hole of agarose gel for electrophoresis.2.It is recommended that the electrophoresis conditions are 2-3 % agarose gel, voltage 4-10V / cm.3.Staining with GelstainRed or other dyes to observe the electrophoretic bands.Product parameters:Ribbon composition：100 bp，200 bp，300 bp，400 bp，500 bp，600bp，700 bp，800 bp，900 bp，1000 bp，1500 bp Scope of application：DNA ladder... Read More | Inquire | Inquire | Inquire | Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleavesRibonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies. ApplicationRibonuclease T1 has extensive applications in molecular cloning and DNA sequencing. Because of its specificity it has been a commonly used cleavage enzyme for the determination of structure, nearest neighbor frequencies, and RNA sequencing. The enzyme has further application in the preparation of nucleoside 2',3'-cyclic phosphates, the synthesis of oligonucleotides, and the removal of RNA from DNA preparations. The enzyme is also used as a non-mammalian source of RNase in various applications... Read More |