| Description | Product contentS665699Component1 mL5 mLStorageS665699A2×Super Kfx MasterMix (Dye)1 mL 5×1 mL -20℃. Avoid freeze/thaw cycle.S665699BddH2O1 mL5×1 mL -20℃. Avoid freeze/thaw cycle.Product IntroductionThis product is a premixed system composed of Super Kfx DNA Polymerase, Mg2+Product contentS665699Component1 mL5 mLStorageS665699A2×Super Kfx MasterMix (Dye)1 mL 5×1 mL -20℃. Avoid freeze/thaw cycle.S665699BddH2O1 mL5×1 mL -20℃. Avoid freeze/thaw cycle.Product IntroductionThis product is a premixed system composed of Super Kfx DNA Polymerase, Mg2+, dNTPs, PCR stabilizers and enhancers, with a concentration of 2 ×. Super Kfx DNA Polymerase is a fast and highly efficient high fidelity DNA polymerase with 5 ′ -3 ′ DNA polymerase activity and 3 ′ -5 ′ exonuclease activity. It has advantages such as strong amplification ability, high fidelity, and strong specificity. The addition of unique amplification enhancers and elongation factors in 2xMix results in a unique formula that makes the entire reaction system very stable, easy to operate, and suitable for amplification of various fragments and templates. This product is suitable for gene cloning, second-generation library amplification, gene directed mutagenesis, SNP amplification experiments, etc. This product has been added with dye (blue), and after the reaction is completed, agarose electrophoresis detection can be directly performed, making the operation convenient and simple. Quality controlAfter testing, there is no exogenous nuclease activity and no residual host DNA, which can effectively amplify various templates. UsageThe following are examples of conventional PCR reaction systems and reaction conditions. In practical operation, corresponding improvements and optimizations should be made based on different templates, primer structures, and target fragment sizes.1. PCR reaction systemAll operations should be carried out on ice. After each group is decomposed and frozen, please mix thoroughly. After use, please put it back at -20 ℃ for storage in a timely manner. 2. PCR reaction conditions Attention:1) Priority should be given to using the three-step method for amplification. If the three-step method cannot amplify the target product or the Tm value of the primer is greater than 68 ° C, please try the two-step method.2) Denaturation: Pre denaturation of simple templates at 98 ℃ for 30 seconds to 1 minute. For complex templates, the pre denaturation time can be extended to 3 minutes.3) Annealing: In general experiments, the annealing temperature is 3-5 ℃ lower than the Tm value of the primer. If the ideal amplification efficiency cannot be achieved, the annealing temperature should be gradually changed for optimization; When non-specific reactions occur, increase the annealing temperature appropriately.4) Extension: The extension time should be set based on the length of the amplified fragment and the complexity of the template. The amplification efficiency of this product is 4-6 kb/min. For long fragments and high complexity templates, it is recommended to set 2-4 kb/min.5) Cycle count: The number of cycles can be set based on the downstream application of the amplification product. If there are too few cycles, insufficient amplification, or too many cycles, the probability of mismatch will increase. Therefore, while ensuring product yield, the number of cycles should be minimized as much as possible... Read More | EPOCROSTM is a reactive polymer with an oxazoline group on the side chain and is used as a cross-linking agent for water-based resins. Among the water-based polymers developed to address environmental issues and the increasing use of water-based products due to VOC regulations and desolventing, the EPOCROSTM is a reactive polymer with an oxazoline group on the side chain and is used as a cross-linking agent for water-based resins. Among the water-based polymers developed to address environmental issues and the increasing use of water-based products due to VOC regulations and desolventing, the EPOCROSTM WS series is a “water-soluble type” with the following structure.Features and PropertiesHigher reactivity than water-based epoxy, melamine, blocked isocyanateVOC free (EPOCROS™ WS-300 and EPOCROS™ WS-700)High crosslinking density with a small amount addedOne-pack type with long usage timeImproves water resistance, solvent resistance, heat resistance, and the strength of films, etc.Adhesion-imparting possible to PET, OPP, PVC, etc.Fast dryingLow toxicity (Ames Test: Negative, Primary Skin Irritation Test: No irritation)WS Series Product LineupApplicationsNonwoven fabric bindersPigment printingCoatings (metals, films, leather)Paint and coatings, Primers (plastics, building materials, vehicles)AdhesivesMethodASSAY for Product Code DILW:One unit equals a decrease in absorbance of 1.0 per minute at 25°C at pH 7.5 with 2,6-dichlorophenolindophenol as the chromogen.Reagents0.2 M Tris⋅HCl buffer, pH 7.50.006 M NADH. Prepare fresh daily.0.0012 M Dichlorophenolindophenol (DCPIP) Prepare fresh daily.EnzymePrepare a 10 mg/ml solution of enzyme in 0.2 M Tris⋅HCl, pH 7.5.Dilute further immediately before use to give ΔA/min of 0.15-0.20.ProcedureAdjust spectrophotometer to 600 nm and 25°C.Pipette into cuvettes as follows:Mix quickly and measure the decrease in absorbance at 600 nm for 2-3 minutes.Determine the ΔA/min. from the initial linear portion of the curve. (Use portion of curve from t=0 to t=1 minute; the rate is linear for 1/2 to 1 minute.)Calculation... Read More | Heme Oxygenase-1-IN-1 (Compound 2) is a heme oxygenase 1 ( HO-1 ) inhibitor with an IC 50 of 0.25 µMIC50& Target:IC 50 : 0.25 µM (HO-1) | Inquire | Purity>97% by SDS-PAGE and HPLC analyses.FunctionPlays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells Purity>97% by SDS-PAGE and HPLC analyses.FunctionPlays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase... Read More |