| Description | Product contentS665699Component1 mL5 mLStorageS665699A2×Super Kfx MasterMix (Dye)1 mL 5×1 mL -20℃. Avoid freeze/thaw cycle.S665699BddH2O1 mL5×1 mL -20℃. Avoid freeze/thaw cycle.Product IntroductionThis product is a premixed system composed of Super Kfx DNA Polymerase, Mg2+Product contentS665699Component1 mL5 mLStorageS665699A2×Super Kfx MasterMix (Dye)1 mL 5×1 mL -20℃. Avoid freeze/thaw cycle.S665699BddH2O1 mL5×1 mL -20℃. Avoid freeze/thaw cycle.Product IntroductionThis product is a premixed system composed of Super Kfx DNA Polymerase, Mg2+, dNTPs, PCR stabilizers and enhancers, with a concentration of 2 ×. Super Kfx DNA Polymerase is a fast and highly efficient high fidelity DNA polymerase with 5 ′ -3 ′ DNA polymerase activity and 3 ′ -5 ′ exonuclease activity. It has advantages such as strong amplification ability, high fidelity, and strong specificity. The addition of unique amplification enhancers and elongation factors in 2xMix results in a unique formula that makes the entire reaction system very stable, easy to operate, and suitable for amplification of various fragments and templates. This product is suitable for gene cloning, second-generation library amplification, gene directed mutagenesis, SNP amplification experiments, etc. This product has been added with dye (blue), and after the reaction is completed, agarose electrophoresis detection can be directly performed, making the operation convenient and simple. Quality controlAfter testing, there is no exogenous nuclease activity and no residual host DNA, which can effectively amplify various templates. UsageThe following are examples of conventional PCR reaction systems and reaction conditions. In practical operation, corresponding improvements and optimizations should be made based on different templates, primer structures, and target fragment sizes.1. PCR reaction systemAll operations should be carried out on ice. After each group is decomposed and frozen, please mix thoroughly. After use, please put it back at -20 ℃ for storage in a timely manner. 2. PCR reaction conditions Attention:1) Priority should be given to using the three-step method for amplification. If the three-step method cannot amplify the target product or the Tm value of the primer is greater than 68 ° C, please try the two-step method.2) Denaturation: Pre denaturation of simple templates at 98 ℃ for 30 seconds to 1 minute. For complex templates, the pre denaturation time can be extended to 3 minutes.3) Annealing: In general experiments, the annealing temperature is 3-5 ℃ lower than the Tm value of the primer. If the ideal amplification efficiency cannot be achieved, the annealing temperature should be gradually changed for optimization; When non-specific reactions occur, increase the annealing temperature appropriately.4) Extension: The extension time should be set based on the length of the amplified fragment and the complexity of the template. The amplification efficiency of this product is 4-6 kb/min. For long fragments and high complexity templates, it is recommended to set 2-4 kb/min.5) Cycle count: The number of cycles can be set based on the downstream application of the amplification product. If there are too few cycles, insufficient amplification, or too many cycles, the probability of mismatch will increase. Therefore, while ensuring product yield, the number of cycles should be minimized as much as possible... Read More | Inquire | Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. Mature human CD4 consists of a 371 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain. Within the ECD, human CD4 shares approximately 52% aa sequence identity with mouse and rat CD4. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity. CD4 is reexpressed on circulating CD8+ T cells upon activation and contributes to their cytotoxic effector activity. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse. CD4 binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1... Read More | Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description: High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2, and HMGB3. HMGB1 is a DNA-binding nuclear protein,Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description: High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2, and HMGB3. HMGB1 is a DNA-binding nuclear protein, released actively following cytokine stimulation as well as passively during cell death. It is the prototypic damage-associated molecular pattern (DAMP) molecule and has been implicated in several inflammatory disorders. HMGB1 signals via the receptor for advanced glycation end-product (RAGE) and members of the toll-like receptor (TLR) family. The most prominent HMGB1 protein and mRNA expression arthritis are present in pannus regions, where synovial tissue invades articular cartilage and bone. HMGB1 promotes the activity of proteolytic enzymes, and osteoclasts need HMGB1 for functional maturation. As a non-histone nuclear protein, HMGB1 has a dual function. Inside the cell, HMGB1 binds DNA, regulating transcription, and determining chromosomal architecture. Outside the cell, HMGB1 can serve as an alarmin to activate the innate system and mediate a wide range of physiological and pathological responses. Extracellular HMGB1 represents an optimal " necrotic marker" selected by the innate immune system to recognize tissue damage and initiate reparative responses. However, extracellular HMGB1 also acts as a potent pro-inflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. HMGB1 has been successfully therapeutically targeted in multiple preclinical models of infectious and sterile diseases including arthritis. As shown in studies on patients as well as animal models, HMGB1 can play an important role in the pathogenesis of the rheumatic disease, including rheumatoid arthritis, systemic lupus erythematosus, and polymyositis among others. Besides, enhanced postmyocardial infarction remodeling in type 1 diabetes mellitus was partially mediated by HMGB1 activation... Read More | Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description:BIRC5, also known as Survivin and EPR-1, is a member of theIAP family. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but BIRC5 has only a single BIR domain. It is expressed cell Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description:BIRC5, also known as Survivin and EPR-1, is a member of theIAP family. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but BIRC5 has only a single BIR domain. It is expressed cell cycle-dependently and highly expressed at mitosis. As a multitasking protein, BIRC5 has dual roles in promoting cell proliferation and preventing apoptosis. Survivin is a component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Survivin acts as an important regulator of the localization of this complex. It may counteract a default induction of apoptosis in G2/M phase... Read More |