| Description | Product contentS665699Component1 mL5 mLStorageS665699A2×Super Kfx MasterMix (Dye)1 mL 5×1 mL -20℃. Avoid freeze/thaw cycle.S665699BddH2O1 mL5×1 mL -20℃. Avoid freeze/thaw cycle.Product IntroductionThis product is a premixed system composed of Super Kfx DNA Polymerase, Mg2+Product contentS665699Component1 mL5 mLStorageS665699A2×Super Kfx MasterMix (Dye)1 mL 5×1 mL -20℃. Avoid freeze/thaw cycle.S665699BddH2O1 mL5×1 mL -20℃. Avoid freeze/thaw cycle.Product IntroductionThis product is a premixed system composed of Super Kfx DNA Polymerase, Mg2+, dNTPs, PCR stabilizers and enhancers, with a concentration of 2 ×. Super Kfx DNA Polymerase is a fast and highly efficient high fidelity DNA polymerase with 5 ′ -3 ′ DNA polymerase activity and 3 ′ -5 ′ exonuclease activity. It has advantages such as strong amplification ability, high fidelity, and strong specificity. The addition of unique amplification enhancers and elongation factors in 2xMix results in a unique formula that makes the entire reaction system very stable, easy to operate, and suitable for amplification of various fragments and templates. This product is suitable for gene cloning, second-generation library amplification, gene directed mutagenesis, SNP amplification experiments, etc. This product has been added with dye (blue), and after the reaction is completed, agarose electrophoresis detection can be directly performed, making the operation convenient and simple. Quality controlAfter testing, there is no exogenous nuclease activity and no residual host DNA, which can effectively amplify various templates. UsageThe following are examples of conventional PCR reaction systems and reaction conditions. In practical operation, corresponding improvements and optimizations should be made based on different templates, primer structures, and target fragment sizes.1. PCR reaction systemAll operations should be carried out on ice. After each group is decomposed and frozen, please mix thoroughly. After use, please put it back at -20 ℃ for storage in a timely manner. 2. PCR reaction conditions Attention:1) Priority should be given to using the three-step method for amplification. If the three-step method cannot amplify the target product or the Tm value of the primer is greater than 68 ° C, please try the two-step method.2) Denaturation: Pre denaturation of simple templates at 98 ℃ for 30 seconds to 1 minute. For complex templates, the pre denaturation time can be extended to 3 minutes.3) Annealing: In general experiments, the annealing temperature is 3-5 ℃ lower than the Tm value of the primer. If the ideal amplification efficiency cannot be achieved, the annealing temperature should be gradually changed for optimization; When non-specific reactions occur, increase the annealing temperature appropriately.4) Extension: The extension time should be set based on the length of the amplified fragment and the complexity of the template. The amplification efficiency of this product is 4-6 kb/min. For long fragments and high complexity templates, it is recommended to set 2-4 kb/min.5) Cycle count: The number of cycles can be set based on the downstream application of the amplification product. If there are too few cycles, insufficient amplification, or too many cycles, the probability of mismatch will increase. Therefore, while ensuring product yield, the number of cycles should be minimized as much as possible... Read More | Inquire | Inquire | Purity>95% SDS-PAGE.FunctionImportant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose Purity>95% SDS-PAGE.FunctionImportant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.Post-translationalHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation... Read More | Inquire |