| Description | Product description：Number of SamplesSufficient for 25 samplesAmount of SamplesUp to 1 nmol of glycolipidSuitable of Samples Isolated glycolipids containing a ceramide backbone.Suggested usage1. Pipette 5 µl of LZ-CER-BUFFX4 into an Eppendorf tube, followed by 5 µl of approximately Product description：Number of SamplesSufficient for 25 samplesAmount of SamplesUp to 1 nmol of glycolipidSuitable of Samples Isolated glycolipids containing a ceramide backbone.Suggested usage1. Pipette 5 µl of LZ-CER-BUFFX4 into an Eppendorf tube, followed by 5 µl of approximately 1nmol of the glycolipid sample to be tested.2. For the positive control reaction, add 5 µl of GLIP-GM1-01 substrate to 5 µl of LZ-CER-BUFFX4 into an Eppendorf tube3. Add 2 µl of LZ-CER-HM-10 (the enzyme) to the reaction mixture and mix gently by tapping the Eppendorf tube with your finger. Make total reaction mix up to 20 µl with water.4. Incubate your samples in a water bath, oven or any other constant heating source at 37°C for 24 hrs.The samples are now ready for procainamide, 2-AB or 2-AA labeling and subsequent analysis by HPLC. Samples can be stored frozen at this point.ApplicationCeramide glycanase is an enzyme used to release glycans from glycosphingolipids (GSLs) to enable their characterisation. It cleaves glycans including GM1, GM2 and GM3 by cleaving the β-glycosyl linkage. Glycans can then be labelled using LudgerTag labelling technology... Read More | Inquire | Inquire | IRE1α kinase-IN-2 is a potent IRE1α kinase inhibitor, with an EC 50 of 0.82 µM. IRE1α kinase-IN-2 inhibits IRE1α kinase autophosphorylation (IC 50 =3.12 µM). IRE1α kinase-IN-2 inhibits XBP1 mRNA splicing in the WT cell lines.In VitroIRE1α kinase-IN-2 (compoundIRE1α kinase-IN-2 is a potent IRE1α kinase inhibitor, with an EC 50 of 0.82 µM. IRE1α kinase-IN-2 inhibits IRE1α kinase autophosphorylation (IC 50 =3.12 µM). IRE1α kinase-IN-2 inhibits XBP1 mRNA splicing in the WT cell lines.In VitroIRE1α kinase-IN-2 (compound 3) inhibits XBP1 mRNA splicing, even during ER stress. MCE has not independently confirmed the accuracy of these methods. They are for reference only.Form:Solid... Read More | Purity>98% SDS-PAGE. purified using conventional chromatography techniques.FunctionChemotactic activity for lymphocytes but not for monocytes or neutrophils.Chemokine (C motif) ligand (XCL1), as known as lymphotactin, is the only known member of the C-chemokine family and signals through the Purity>98% SDS-PAGE. purified using conventional chromatography techniques.FunctionChemotactic activity for lymphocytes but not for monocytes or neutrophils.Chemokine (C motif) ligand (XCL1), as known as lymphotactin, is the only known member of the C-chemokine family and signals through the receptor XCR1, formally known as GPR5. The expression of lymphotactin is abundant in some activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces its chemotactic function by binding to a chemokine receptor called XCR1. Recombinant Human XCL1 which is a single non-glycosylated polypeptide chains containing 92 amino acids and it shares approximately 60 % amino acid sequence homology with the murine and rat protein... Read More |