| Description | Product description：Number of SamplesSufficient for 25 samplesAmount of SamplesUp to 1 nmol of glycolipidSuitable of Samples Isolated glycolipids containing a ceramide backbone.Suggested usage1. Pipette 5 µl of LZ-CER-BUFFX4 into an Eppendorf tube, followed by 5 µl of approximately Product description：Number of SamplesSufficient for 25 samplesAmount of SamplesUp to 1 nmol of glycolipidSuitable of Samples Isolated glycolipids containing a ceramide backbone.Suggested usage1. Pipette 5 µl of LZ-CER-BUFFX4 into an Eppendorf tube, followed by 5 µl of approximately 1nmol of the glycolipid sample to be tested.2. For the positive control reaction, add 5 µl of GLIP-GM1-01 substrate to 5 µl of LZ-CER-BUFFX4 into an Eppendorf tube3. Add 2 µl of LZ-CER-HM-10 (the enzyme) to the reaction mixture and mix gently by tapping the Eppendorf tube with your finger. Make total reaction mix up to 20 µl with water.4. Incubate your samples in a water bath, oven or any other constant heating source at 37°C for 24 hrs.The samples are now ready for procainamide, 2-AB or 2-AA labeling and subsequent analysis by HPLC. Samples can be stored frozen at this point.ApplicationCeramide glycanase is an enzyme used to release glycans from glycosphingolipids (GSLs) to enable their characterisation. It cleaves glycans including GM1, GM2 and GM3 by cleaving the β-glycosyl linkage. Glycans can then be labelled using LudgerTag labelling technology... Read More | Heme Oxygenase-1-IN-1 (Compound 2) is a heme oxygenase 1 ( HO-1 ) inhibitor with an IC 50 of 0.25 µMIC50& Target:IC 50 : 0.25 µM (HO-1) | Lipase PS is generally used in the enantioselective transesterification and hydrolysis. Applications include: 1.Lipase catalyzed transesterification of prochiral pyrimidine acyclonucleoside. 2.Lipase catalyzed hydrolysis of diacetylated pyrimidine acyclonucleosides. 3. Enantiomer selective acylationLipase PS is generally used in the enantioselective transesterification and hydrolysis. Applications include: 1.Lipase catalyzed transesterification of prochiral pyrimidine acyclonucleoside. 2.Lipase catalyzed hydrolysis of diacetylated pyrimidine acyclonucleosides. 3. Enantiomer selective acylation of racemic alcohols in continuous-flow bioreactors... Read More | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Histones are a complex family of highly conserved basic proteins responsible for packaging chromosomal DNA into nucleosomes. Histone proteins exhibit two levels of diversity: 1. evolutionary diversity Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Histones are a complex family of highly conserved basic proteins responsible for packaging chromosomal DNA into nucleosomes. Histone proteins exhibit two levels of diversity: 1. evolutionary diversity between species and 2. subtype diversity in a class(H1, H2A, H2B, H3 or H4) within a species. It has become more and more evident that histone modifications are key players in the regulation of chromatin states and dynamics as well as in gene expression. Therefore, histone modifications and the enzymatic machinery that set them are crucial regulators that can control cellular proliferation, differentiation, plasticity, and malignancy processes. However, extracellular histones are a double-edged sword because they also damage host tissue and may cause death. Histones bound to platelets, induced calcium influx, and recruited plasma adhesion proteins such as fibrinogen to induce platelet aggregation. Histone H2B proteins have been studied in a variety of species and are easily detected in most species. The reversible ubiquitylation of histone H2B has long been implicated in transcriptional activation and gene silencing. Phosphorylation of H2B serine 32 occurs in normal cycling and mitogen-stimulated cells. Notably, this phosphorylation is elevated in skin cancer cell lines and tissues compared with normal counterparts. HIST2H2BE is a member of the histone H2B family and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif... Read More | Inquire |