| Description | 1、Product attributeShelf life: 36 monthsMarker enzyme: Horseradish peroxidase (HRP)Reaction time：short (up to 20 minutes) at 20-25°CLot-to-lot variation：< 10 %Boiling point : 100℃（Calculated flash point）Flash point:：120 °C （1、Product attributeShelf life: 36 monthsMarker enzyme: Horseradish peroxidase (HRP)Reaction time：short (up to 20 minutes) at 20-25°CLot-to-lot variation：< 10 %Boiling point : 100℃（Calculated flash point）Flash point:：120 °C （Calculated flash point）pH-Value (at 20 °C): 4.5-4.9 （Experimental data）Density (20℃) : 1.0064 g/cm³Water solubility: easily solubleAppearance: colourless liquidOdour: odourlessLight sensitiveHeat sensitive2、Requirements for storage rooms and vessels1.Keep container tightly closed.2.Keep cool. Protect from sunlight.3.Keep/Store only in original container.4.Never return spills in original containers for reuse.5. Keep away from: Food and feedingstuffs.6. The solution can be transported at room temperature, but temperatures exceeding 30 °C have to be avoided. Shipping should be completed within one week.7. The solution will still work beyond the expiry date, but a lower sensitivity has to be taken into account and a precipitate may occur.8. Contaminated or leaked out substrate solution from damaged bottles should not be used and has to be destroyed.3、Effective Components and Principle of FunctionA citrate buffer system (pH 4.5 - 5.0) contains the following effective components:Hydrogen peroxide3,3`,5,5`-Tetramethylbenzidine (TMB)precipitating reagentPeroxidase catalyses the decomposition of hydrogen peroxide, taking electrons from TMB (byoxidation). Oxidation of TMB forms a radical cation that stabilizes by dimerisation and shows the typical blue colour. This blue cation is stabilized by forming an insoluble complex with the precipitating reagent. So at positions with horseradish peroxidase activity a blue precipitate will appear. This precipitate is very fine so that even the smallest dots can be coloured regularly.4、Biosafety informationThis mixture is not classified as hazardous in accordance with Regulation (EC) No 1272/2008；Hazardous components：none (according to Regulation (EC) No 1907/2006 (REACH))5、Advantage1. Rapid precipitation2. Strong adherence to plastic surfaces3. Short reaction time4. Intensive dark blue colour5. Homogeneous staining pattern6、Instruction for usageFor bottling consider the following instructions:•Work in a dust free and darkened room.• Pay attention that the solution has no contact with metal parts (leading to catalysis) of yourinstruments. Closed systems of silicon tubes are favoured.• Clean all instruments and vessels very carefully.• Never touch parts of the instruments that are in contact with the solution with the naked hand. Wear powder free gloves.• Close the bottles immediately to minimize the influence of light and dust.• Use bottles that are not permeable to light, made from HDPE or PP.7. General Instructions for the Use on Microchips• This solution should only be used by qualified laboratory staff familiar with the basics of immunological methods.• Note: The substrate solution is very sensitive to impurities. This is why you should never immerse pipette tips in storage bottles. Unused solution is never returned to the bottle! Don't swap the bottles for the cups!• Substrate Solutions Test systems for instrumental measurements on glass or plastic surfaces (polycarbonate or polystyrene).• Depending on the system, it may be sufficient to wash the microarrays after incubation with the conjugate and then cover them with the substrate solution. Arrays can be developed with a rocking motion until visible color appears (approximately 5 to 10 minutes). Measurements can be performed using wet arrays or after washing with distilled water and drying. Dry microchips can be used for recording purposes. (Note: some glues will destroy colored deposits)... Read More | Protein Purity≥85% by SDS PAGEExtinction CoeffA280 nm = 0.974 at 1.0 mg/ml for pure C3bMolecular Weight185,000 Da (2 chains)General DescriptionCynomolgus monkey C3 (cyno C3) is purified from pooled normal cynomolgus monkey serum. C3 is central to the activation of all three pathways of Protein Purity≥85% by SDS PAGEExtinction CoeffA280 nm = 0.974 at 1.0 mg/ml for pure C3bMolecular Weight185,000 Da (2 chains)General DescriptionCynomolgus monkey C3 (cyno C3) is purified from pooled normal cynomolgus monkey serum. C3 is central to the activation of all three pathways of complement activation (Law, S.K.A. and Reid, K.B.M. (1995)). Initiation of each pathway generates proteolytic enzyme complexes (C3 convertases) which are bound to the target surface. These enzymes cleave a peptide bond in C3 releasing the anaphylatoxin C3a and activating C3b. For a brief time (~60 µs) this nascent C3b is capable of reacting with and covalently coupling to hydroxyl groups on the target surface. Carbohydrates are the favored target, but protein hydroxyls and amino groups also react. This process of tagging the target surface with C3b is called opsonization. The reactive site in nascent C3b is a thioester (Tack B.J., et al. (1980); Pangburn M.K. and MüllerEberhard H.J. (1980)) and C3b is linked to the target through a covalent ester bond (an amide bond is formed if C3b is attached to amino groups). Most of the C3 activated during complement activation never attaches to the surface because its thioester reacts with water forming fluid phase C3b which is rapidly inactivated by factors H and I forming iC3b. Surface-bound C3b is necessary in all three pathways for efficient activation of C5 and formation of C5b-9 complexes that lyse the target cell membrane. Surface-bound C3b and its breakdown products iC3b and C3d are recognized by numerous receptors on lymphoid and phagocytic cells which use the C3b ligand to stimulate antigen presentation to cells of the adaptive immune system. The end result is an expansion of target-specific B-cell and T-cell populations.Physical Characteristics & StructureCynomolgus monkey C3 is an uncharacterized protein. The calculated molecular weight based on its amino acid sequence is 184,926 daltons similar to that of human C3 (185,000 daltons). Like human C3, cyno C3 is composed of two disulfide-linked chains. Analysis of purified cyno C3 by SDS/polyacrylamide gel electrophoresis under non-reduced conditions shows the mobility of cyno C3 to be similar to that of human C3. Under reduced conditions, the migration of the alpha chain of cyno C3 is comparable to that of human C3 alpha chain (110,000 daltons) while the beta chain migrates slightly ahead of the human C3 beta chain (75,000daltons).The extinction coefficient of cyno C3 is calculated from its amino acid sequence using ProtParam and assumes all pairs of Cys residues form cystines (i.e. a pair of cystine molecules are joined by a disulfide bond). The theoretical pI value for cyno monkey C3 is 6.03. Employing immunoturbidimetric method the serum concentration of cyno C3 has been reported to be 1.27 mg/ml in males and 1.1 mg/ml in female monkeys (Park H-K et al., (2016)). FunctionThe biological functions of C3 are described above in the General Description and Physical Characteristics sections.GeneticsCynomolgus monkey C3 chromosome location 19. The NCBI Gene ID number for Cynomolgus monkey C3 is 102131458 and UniProt accession number is A0A2K5VPN1.Precautions/Toxicity/HazardsThis protein is purified from animal serum and therefore precautions appropriate for handling any animal blood-derived product must be used.ReferencesLaw, S.K.A. and Reid, K.B.M. (1995) Complement 2nd Edition (ISBN 0199633568) Oxford University Press, Oxford.Tack BF, Harrison RA, Janatova J, Thomas ML, Prahl JW. (1980) Evidence for presence of an internal thiolester bond in third component of human complement. Proc Natl Acad Sci U S A. 77:5764-8.Pangburn M.K. and Müller-Eberhard H.J. (1980) Relation of putative thioester bond in C3 to activation of the alternative pathway and the binding of C3b to biological targets of complement. J Exp Med. 152:1102-14.Park H-K, Cho J-W, Lee B-S, Park H, Han J-S, Yang M-J, Im W-J, Park D-Y, Kim W-J, Han SC, Kim Y-B. (2016) Reference values of clinical pathology parameters in cynomolgus monkeys (Macaca fascicularis) used in preclinical studies. Lab Anim Res. 32(2):79-86... Read More | Inquire | Purity>95% SDS-PAGE.FunctionB Cell Activating Factor Receptor (BAFF-R), also named tumor necrosis factor receptor superfamily member 13C, is a member of the TNFR superfamily. It is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ Purity>95% SDS-PAGE.FunctionB Cell Activating Factor Receptor (BAFF-R), also named tumor necrosis factor receptor superfamily member 13C, is a member of the TNFR superfamily. It is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFF receptor is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain and binds with high specificity to BAFF (TNFSF13B). It enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF receptor/BAFF signaling plays a critical role in B cell survival and maturation... Read More | Inquire |