| Quantity | 250ml, 25ml, 500ml, 50ml, 1ml, 5ml, 100ml | 50ml, 250ml, 10ml | 200T | 50mg, 10mg, 100mg, 5mg, 25mg, 1mg | 100µg, 10µg, 50µg, 1mg |
| Description | 1、Product attributeShelf life: 36 monthsMarker enzyme: Horseradish peroxidase (HRP)Reaction time：short (up to 20 minutes) at 20-25°CLot-to-lot variation：< 10 %Boiling point : 100℃（Calculated flash point）Flash point:：120 °C （1、Product attributeShelf life: 36 monthsMarker enzyme: Horseradish peroxidase (HRP)Reaction time：short (up to 20 minutes) at 20-25°CLot-to-lot variation：< 10 %Boiling point : 100℃（Calculated flash point）Flash point:：120 °C （Calculated flash point）pH-Value (at 20 °C): 4.5-4.9 （Experimental data）Density (20℃) : 1.0064 g/cm³Water solubility: easily solubleAppearance: colourless liquidOdour: odourlessLight sensitiveHeat sensitive2、Requirements for storage rooms and vessels1.Keep container tightly closed.2.Keep cool. Protect from sunlight.3.Keep/Store only in original container.4.Never return spills in original containers for reuse.5. Keep away from: Food and feedingstuffs.6. The solution can be transported at room temperature, but temperatures exceeding 30 °C have to be avoided. Shipping should be completed within one week.7. The solution will still work beyond the expiry date, but a lower sensitivity has to be taken into account and a precipitate may occur.8. Contaminated or leaked out substrate solution from damaged bottles should not be used and has to be destroyed.3、Effective Components and Principle of FunctionA citrate buffer system (pH 4.5 - 5.0) contains the following effective components:Hydrogen peroxide3,3`,5,5`-Tetramethylbenzidine (TMB)precipitating reagentPeroxidase catalyses the decomposition of hydrogen peroxide, taking electrons from TMB (byoxidation). Oxidation of TMB forms a radical cation that stabilizes by dimerisation and shows the typical blue colour. This blue cation is stabilized by forming an insoluble complex with the precipitating reagent. So at positions with horseradish peroxidase activity a blue precipitate will appear. This precipitate is very fine so that even the smallest dots can be coloured regularly.4、Biosafety informationThis mixture is not classified as hazardous in accordance with Regulation (EC) No 1272/2008；Hazardous components：none (according to Regulation (EC) No 1907/2006 (REACH))5、Advantage1. Rapid precipitation2. Strong adherence to plastic surfaces3. Short reaction time4. Intensive dark blue colour5. Homogeneous staining pattern6、Instruction for usageFor bottling consider the following instructions:•Work in a dust free and darkened room.• Pay attention that the solution has no contact with metal parts (leading to catalysis) of yourinstruments. Closed systems of silicon tubes are favoured.• Clean all instruments and vessels very carefully.• Never touch parts of the instruments that are in contact with the solution with the naked hand. Wear powder free gloves.• Close the bottles immediately to minimize the influence of light and dust.• Use bottles that are not permeable to light, made from HDPE or PP.7. General Instructions for the Use on Microchips• This solution should only be used by qualified laboratory staff familiar with the basics of immunological methods.• Note: The substrate solution is very sensitive to impurities. This is why you should never immerse pipette tips in storage bottles. Unused solution is never returned to the bottle! Don't swap the bottles for the cups!• Substrate Solutions Test systems for instrumental measurements on glass or plastic surfaces (polycarbonate or polystyrene).• Depending on the system, it may be sufficient to wash the microarrays after incubation with the conjugate and then cover them with the substrate solution. Arrays can be developed with a rocking motion until visible color appears (approximately 5 to 10 minutes). Measurements can be performed using wet arrays or after washing with distilled water and drying. Dry microchips can be used for recording purposes. (Note: some glues will destroy colored deposits)... Read More | Inquire | This product is a mixture of fast reverse transcription reagents. The 5 x EasyQuick RT MasterMix contains all the reagents required for reverse transcription from RNA templates to cDNA first strand, including EasyQuick RT Reversase, RNase Inhibitor, Random 6 mers, Oligo dT Primer, dNTP, EQ-RT BufferThis product is a mixture of fast reverse transcription reagents. The 5 x EasyQuick RT MasterMix contains all the reagents required for reverse transcription from RNA templates to cDNA first strand, including EasyQuick RT Reversase, RNase Inhibitor, Random 6 mers, Oligo dT Primer, dNTP, EQ-RT Buffer, etc. The reverse transcription efficiency of this product is high, and it can perform a good reverse transcription reaction on a small amount of RNA templates. The fluorescence quantitative template cDNA first strand synthesis can be completed in 15 minutes. This reagent kit is very convenient and fast to operate, and only RNA templates and water need to be added for reverse transcription reaction, making it particularly suitable for high-throughput detection.E665905Component200 TStorageE665905A5×EasyQuick RT MasterMix 400 µL-20℃. Avoid freeze/thaw cycle.E665905BRNase-Free Water 2×1 mL-20℃. Avoid freeze/thaw cycle. Product features1. Convenience: The ready to use reverse transcription Mix only requires the addition of RNA templates and water to initiate the reaction.2. Fast: Complete cDNA first strand synthesis in 15 minutes.3. High reverse transcription efficiency: The reverse transcription efficiency is above 90%.4. High sensitivity: PG level templates can also obtain high-quality cDNA.5. Read through complex templates: templates with high GC content and complex secondary structures.Matters needing attention1. During the operation, RNase contamination should be avoided to prevent RNA degradation or cross contamination during experiments. It is recommended that operators wear masks and disposable gloves, frequently change gloves, and use specialized instruments and consumables.2. The reverse transcription system is prepared on ice for operation to prevent RNA degradation. The MasterMix of the reagent kit should be stored at -20 ℃ as soon as possible after use, and repeated freeze-thaw should be avoided as much as possible.3.10 µ The reaction system can be used up to 1 µ G Total RNA, if the amount of template RNA is greater than 1 µ g. Please expand the reaction system proportionally.4. For RNA templates with complex secondary structures, it is recommended to incubate the template RNA at 65 ℃ for 5 minutes on ice before proceeding with the next step, followed by brief centrifugation.Operation steps1. Thaw the template RNA on ice; After thawing the components of the reagent kit at room temperature, immediately place them on ice. Before use, vortex shake and mix each solution, and centrifuge briefly before use.2. Prepare the reaction system according to the following table (please prepare the reaction solution on ice), vortex shake and mix well, briefly centrifuge, and collect the solution on the tube wall to the bottom of the tube. Reagent 10 µl Reaction system Final concentration RNA Template X µl 1 pg～0.5 µg ¹⁾ 5×EasyQuick RT MasterMix ²⁾ 2 µl 1× RNase-Free Water up to 10 µl /Attention:1) If the total RNA content is greater than 1 µ g, please expand the reaction system proportionally.2) 5 x EasyQuick RT MasterMix contains Oligo (dT), Random Prime, RNase Inhibitor, dNTP Mixture, EQ-RT Buffer, etc. 3. Incubate at 37 ℃ for 15 minutes. 4. Incubate at 85 ℃ for 5 seconds to inactivate reverse transcriptase.5. After a brief centrifugation, place it on ice for subsequent experiments. If it needs to be stored for a long time, please place it at -20 ℃... Read More | Inquire | Purity> 95 % by SDS-PAGE and HPLC analyses.FunctionOsteoprotegerin (OPG), also named osteoclastogenesis inhibitory factor (OCIF), and tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), is a TNFRSF11B-encoded protein in humans. Acts as decoy receptor for RANKL and thereby Purity> 95 % by SDS-PAGE and HPLC analyses.FunctionOsteoprotegerin (OPG), also named osteoclastogenesis inhibitory factor (OCIF), and tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), is a TNFRSF11B-encoded protein in humans. Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.OPG has been applied to decrease bone resorption in women with postmenopausal osteoporosis and in patients with lytic bone metastases... Read More |