| Description | The anti fluorescence quenching sealing agent is a kind of reagent that can slow down the fluorescence quenching and cure the glass slide to form a permanent hard seal. The cured glass slide does not need to use nail polish or other sealant to seal the edge of the cover glass slide; DAPI containing The anti fluorescence quenching sealing agent is a kind of reagent that can slow down the fluorescence quenching and cure the glass slide to form a permanent hard seal. The cured glass slide does not need to use nail polish or other sealant to seal the edge of the cover glass slide; DAPI containing antifluorescent quench blocking agent can directly stain nuclei.Instruction：Note : Before use, return it to room temperature and mix well with slight oscillation to avoid bubbles. 1.Cell samples : ( 1 ) After dyeing, absorb the liquid ; ( 2 ) Add 10-20 µL of sealing agent to each glass slide, cover the cover glass with cells, so that the cells can fully contact the sealing agent, and slowly cover the cover glass to seal the operation. The tablets dizzy naturally, try not to have bubbles ; ( 3 ) Fluorescence microscope observation ; ( 4 ) After the end of the observation, placed at 4 °C or room temperature, overnight sealing, pay attention to avoid light. Note : In order to prevent the formation of bubbles, the sealing dose can be appropriately increased. For example, 100-200 µL can be added dropwise to the large cover glass, and then placed in an oven at 37 °C for 1-2 h to accelerate the curing.2.Tissue sections : ( 1 ) After the staining is completed, the staining solution is sucked off ; ( 2 ) Dropping 10-20 µL of anti-fluorescence quenching sealing agent on the tissue section, covering the cover glass to allow the tissue to fully contact the sealing agent, and slowly covering the cover glass during operation to make the sealing agent naturally dizzy, try not to have bubbles ; ( 3 ) Observation of tissue sections by fluorescence microscope ; ( 4 ) After the end of the observation, placed at 4 °C or room temperature, overnight sealing, pay attention to avoid light. Note : In order to prevent the formation of bubbles, the sealing dose can be appropriately increased. For example, 100-200 µL can be added dropwise to the large cover glass, and then placed in an oven at 37 °C for 1-2 h to accelerate the curing. 3.Other samples : Other samples can be operated with reference to the above samples.Matters needing attention：1. the anti fluorescence quenching blocker has poor anti quenching effect on staining live cell membrane dyes and mitochondrial dyes. It is recommended that the anti fluorescence quenching blocker be used to fix permeabilized cells or tissues. 2. anti fluorescence quenching tablet sealer and our YF ® 488 when matching dyes, background interference may occur, which may be caused by adding too much of this product. The amount of this product should be minimized. 3. the anti fluorescence quenching chip sealer may not be suitable for some dyes, so it is recommended to conduct pre experiment to test the matching. 4. during the film sealing process, avoid places with too much moisture. If the anti fluorescence quenching film sealing agent absorbs too much moisture, it will affect the film sealing effect. 5. for your safety and health, please wear experimental clothes and disposable gloves.Scope of application：Antifluorescent quencher... Read More | 2x Taq MasterMix is a premixed system composed of Taq DNA Polymerase, Mg2+, dNTPs, PCR stabilizers, and enhancers. The pre prepared PCR mixture makes the operation simpler and faster, and can minimize human error and contamination to the greatest extent possible. The original MasterMix formula 2x Taq MasterMix is a premixed system composed of Taq DNA Polymerase, Mg2+, dNTPs, PCR stabilizers, and enhancers. The pre prepared PCR mixture makes the operation simpler and faster, and can minimize human error and contamination to the greatest extent possible. The original MasterMix formula results in high yield, strong repeatability, and good stability of amplified products. This product does not contain dyes. After the PCR program is completed, an appropriate amount of sample buffer can be added as needed for electrophoresis operation. The amplified PCR product has an "A" base attached to the 3 'end, making it suitable for direct use in T/A cloning. Mainly suitable for PCR amplification of DNA, DNA sequencing and other experiments.Quality control: T665627Component5mlStorageT665627A2×Taq MasterMix5×1ml-20℃. Avoid freeze/thaw cycle.T665627BddH₂O5×1ml-20℃. Avoid freeze/thaw cycle.Notes: 2×Taq MasterMix contains Taq DNA Polymerase, 3mM MgCl2 and 400µM each dNTP After testing, there was no exogenous nuclease activity; PCR method for detecting residual DNA without host; Can effectively amplify single copy genes from multiple genomes.Usage:The following is an example of a PCR reaction system and reaction conditions for amplifying a 1 kb fragment using human genomic DNA as a template. In practical operation, corresponding improvements and optimizations should be made based on the template, primer structure, and target fragment size.1. PCR reaction systemReagent50 µlReaction systemFinal concentration2×Taq MasterMix25 µl1×Forward Primer，10 µM2 µl0.4 µMReverse Primer，10 µM2 µl0.4 µMTemplate DNA<0.5 µg<0.5 µg/50 µlddH2Oup to 50 µl/Attention: The primer concentration should be between 0.1 and 1.0 as the final concentration µ M serves as a reference for setting the range. In the case of low amplification efficiency, the concentration of primers can be increased; When non-specific reactions occur, the primer concentration can be reduced to optimize the reaction system.2. PCR reaction conditionsStepTemperatureTime/Pre denaturation95℃2 min/Denaturation94℃30 s25-35 cyclesAnneal55-65℃30 s25-35 cyclesExtend72℃30 s25-35 cyclesFinally extended72℃2 min/Attention:1) In general experiments, if the annealing temperature is 5 ℃ lower than the melting temperature Tm of the amplification primer, and the ideal amplification efficiency cannot be achieved, the annealing temperature should be appropriately reduced; When non-specific reactions occur, increase the annealing temperature to optimize the reaction conditions.2) The extension time should be set according to the size of the amplified fragment. The amplification efficiency of Taq DNA Polymerase in this product is 2 kb/min.3) The number of cycles can be set based on the downstream application of the amplification product. If the number of cycles is too small, the amplification amount is insufficient; If there are too many cycles, the probability of mismatches will increase, and non-specific backgrounds will be severe. So, while ensuring product yield, the number of cycles should be minimized as much as possible... Read More | Inquire | Product introduction：The additive contains a variety of cytokines, human albumin and other components. In order to ensure the activity, it is recommended that the additive part be thawed once and then sub packaged and frozen again. It is not advisable to freeze and thaw repeatedly. The Product introduction：The additive contains a variety of cytokines, human albumin and other components. In order to ensure the activity, it is recommended that the additive part be thawed once and then sub packaged and frozen again. It is not advisable to freeze and thaw repeatedly. The product contains no animal serum or animal serum components, no animal protein components, and no antibiotics.Matters needing attention：1. try to reduce the number of repeated freezing and thawing to avoid efficiency decline. 2. it is not suitable to place it at room temperature for a long time. 3. pay attention to aseptic operation and try to avoid pollution. 4. please wear experimental clothes and disposable gloves for operationScope of application：Cell culture additives... Read More | Purity>95% SDS-PAGE.FunctionProbable cell adhesion protein |