| Description | The anti fluorescence quenching sealing agent is a kind of reagent that can slow down the fluorescence quenching and cure the glass slide to form a permanent hard seal. The cured glass slide does not need to use nail polish or other sealant to seal the edge of the cover glass slide; DAPI containing The anti fluorescence quenching sealing agent is a kind of reagent that can slow down the fluorescence quenching and cure the glass slide to form a permanent hard seal. The cured glass slide does not need to use nail polish or other sealant to seal the edge of the cover glass slide; DAPI containing antifluorescent quench blocking agent can directly stain nuclei.Instruction：Note : Before use, return it to room temperature and mix well with slight oscillation to avoid bubbles. 1.Cell samples : ( 1 ) After dyeing, absorb the liquid ; ( 2 ) Add 10-20 µL of sealing agent to each glass slide, cover the cover glass with cells, so that the cells can fully contact the sealing agent, and slowly cover the cover glass to seal the operation. The tablets dizzy naturally, try not to have bubbles ; ( 3 ) Fluorescence microscope observation ; ( 4 ) After the end of the observation, placed at 4 °C or room temperature, overnight sealing, pay attention to avoid light. Note : In order to prevent the formation of bubbles, the sealing dose can be appropriately increased. For example, 100-200 µL can be added dropwise to the large cover glass, and then placed in an oven at 37 °C for 1-2 h to accelerate the curing.2.Tissue sections : ( 1 ) After the staining is completed, the staining solution is sucked off ; ( 2 ) Dropping 10-20 µL of anti-fluorescence quenching sealing agent on the tissue section, covering the cover glass to allow the tissue to fully contact the sealing agent, and slowly covering the cover glass during operation to make the sealing agent naturally dizzy, try not to have bubbles ; ( 3 ) Observation of tissue sections by fluorescence microscope ; ( 4 ) After the end of the observation, placed at 4 °C or room temperature, overnight sealing, pay attention to avoid light. Note : In order to prevent the formation of bubbles, the sealing dose can be appropriately increased. For example, 100-200 µL can be added dropwise to the large cover glass, and then placed in an oven at 37 °C for 1-2 h to accelerate the curing. 3.Other samples : Other samples can be operated with reference to the above samples.Matters needing attention：1. the anti fluorescence quenching blocker has poor anti quenching effect on staining live cell membrane dyes and mitochondrial dyes. It is recommended that the anti fluorescence quenching blocker be used to fix permeabilized cells or tissues. 2. anti fluorescence quenching tablet sealer and our YF ® 488 when matching dyes, background interference may occur, which may be caused by adding too much of this product. The amount of this product should be minimized. 3. the anti fluorescence quenching chip sealer may not be suitable for some dyes, so it is recommended to conduct pre experiment to test the matching. 4. during the film sealing process, avoid places with too much moisture. If the anti fluorescence quenching film sealing agent absorbs too much moisture, it will affect the film sealing effect. 5. for your safety and health, please wear experimental clothes and disposable gloves.Scope of application：Antifluorescent quencher... Read More | Inquire | 1、Product attributeReaction time：short (up to 20 minutes) at 20-37°CLot-to-lot variation：<5%Boiling point : 100℃pH-Value (at 20 °C): 3.5-4.0Density (20℃) : 1.0111 g/cm³Appearance: colourless to pale blue liquidOdour: odourlessRecommend Incubation 1、Product attributeReaction time：short (up to 20 minutes) at 20-37°CLot-to-lot variation：<5%Boiling point : 100℃pH-Value (at 20 °C): 3.5-4.0Density (20℃) : 1.0111 g/cm³Appearance: colourless to pale blue liquidOdour: odourlessRecommend Incubation temperature: 20-37 °C2、Requirements for storage rooms and vessels1.Keep container tightly closed.2.Keep cool. protected from light3.Keep/Store only in original container.4.Never return spills in original containers for reuse.5. Keep away from: Food and feeding stuffs3、It is a ready-to-use, labelling-free TMB-substrate solution.4、Biosafety informationThis mixture is not classified as hazardous in accordance with Regulation (EC) No 1272/2008；5、Advantage1. Very high absorbance yield2. Very low background signals3. Certified long-term stability4. Regeneration following light exposure... Read More | Es Taq DNA Polymerase is an optimized mixed enzyme of Taq and Pfu DNA Polymerase, with 5 '→ 3' DNA polymerase activity, 5 '→ 3' exonuclease activity, and 3 '→ 5' exonuclease activity. Compared with Taq DNA Polymerase, Es Taq DNA Polymerase has excellent performance of high Es Taq DNA Polymerase is an optimized mixed enzyme of Taq and Pfu DNA Polymerase, with 5 '→ 3' DNA polymerase activity, 5 '→ 3' exonuclease activity, and 3 '→ 5' exonuclease activity. Compared with Taq DNA Polymerase, Es Taq DNA Polymerase has excellent performance of high amplification efficiency and low mismatch rate, and can efficiently amplify DNA fragments. Most of the PCR products amplified with this product contain an "A" base at the 3 'end, which can be directly used for T/A cloning. This product is suitable for conventional PCR reactions and gene cloning reactions that require high fidelity. E665597Component500 UStorageE665597AEs Taq DNA Polymerase, 5 U/µL 100 µL -20℃. Avoid freeze/thaw cycle.E665597B10×PCR Buffer 1.8 mL -20℃. Avoid freeze/thaw cycle.Activity definition:Using activated salmon sperm DNA as a template/primer, the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into acidic insoluble substances is defined as 1 active unit (U) at 74 ℃ for 30 minutes.Quality control:After multiple column purifications, SDS-PAGE detected a purity of over 99%; No exogenous nuclease activity detected; PCR method for detecting residual DNA without host; Can effectively amplify single copy genes in the human genome; Store at room temperature for one month without significant changes in activity.1. PCR reaction system Reagent 50 µlReaction system Final concentration 10×PCR Buffer 5 µL 1× dNTP Mix，10 mM each 1 µL 200 µM each Forward Primer，10 µM 2 µL 0.4 µM Reverse Primer，10 µM 2 µl 0.4 µM Template DNA <0.5 µg <0.5 µg/50 µl Es Taq DNA Polymerase，5 U/µl 0.25-0.5 µl 1.25-2.5U/50 µl ddH2O up to 50 µL /Attention: The primer concentration should be between 0.1 and 1.0 as the final concentration µ M serves as a reference for setting the range. In the case of low amplification efficiency, the concentration of primers can be increased; When non-specific reactions occur, the primer concentration can be reduced to optimize the reaction system. 2. PCR reaction conditions Step Temperature Time / Pre denaturation 94℃ 2 min / Denaturation 94℃ 30 s 25-35 cycles Anneal 55-65℃ 30 s 25-35 cycles Extend 72℃ 30 s 25-35 cycles Finally extended 72℃ 2 min / Attention:1) In general experiments, if the annealing temperature is 5 ℃ lower than the melting temperature Tm of the amplification primer, and the ideal amplification efficiency cannot be achieved, the annealing temperature should be appropriately reduced; When non-specific reactions occur, increase the annealing temperature to optimize the reaction conditions.2) The extension time should be set according to the size of the amplified fragment. The amplification efficiency of Es Taq DNA Polymerase in this product is 2 kb/min.3) The number of cycles can be set based on the downstream application of the amplification product. If the number of cycles is too small, the amplification amount is insufficient; If there are too many cycles, the probability of mismatches will increase, and non-specific backgrounds will be severe. So, while ensuring product yield, the number of cycles should be minimized as much as possible... Read More | Inquire |