| Description | The anti fluorescence quenching sealing agent is a kind of reagent that can slow down the fluorescence quenching and cure the glass slide to form a permanent hard seal. The cured glass slide does not need to use nail polish or other sealant to seal the edge of the cover glass slide; DAPI containing The anti fluorescence quenching sealing agent is a kind of reagent that can slow down the fluorescence quenching and cure the glass slide to form a permanent hard seal. The cured glass slide does not need to use nail polish or other sealant to seal the edge of the cover glass slide; DAPI containing antifluorescent quench blocking agent can directly stain nuclei.Instruction：Note : Before use, return it to room temperature and mix well with slight oscillation to avoid bubbles. 1.Cell samples : ( 1 ) After dyeing, absorb the liquid ; ( 2 ) Add 10-20 µL of sealing agent to each glass slide, cover the cover glass with cells, so that the cells can fully contact the sealing agent, and slowly cover the cover glass to seal the operation. The tablets dizzy naturally, try not to have bubbles ; ( 3 ) Fluorescence microscope observation ; ( 4 ) After the end of the observation, placed at 4 °C or room temperature, overnight sealing, pay attention to avoid light. Note : In order to prevent the formation of bubbles, the sealing dose can be appropriately increased. For example, 100-200 µL can be added dropwise to the large cover glass, and then placed in an oven at 37 °C for 1-2 h to accelerate the curing.2.Tissue sections : ( 1 ) After the staining is completed, the staining solution is sucked off ; ( 2 ) Dropping 10-20 µL of anti-fluorescence quenching sealing agent on the tissue section, covering the cover glass to allow the tissue to fully contact the sealing agent, and slowly covering the cover glass during operation to make the sealing agent naturally dizzy, try not to have bubbles ; ( 3 ) Observation of tissue sections by fluorescence microscope ; ( 4 ) After the end of the observation, placed at 4 °C or room temperature, overnight sealing, pay attention to avoid light. Note : In order to prevent the formation of bubbles, the sealing dose can be appropriately increased. For example, 100-200 µL can be added dropwise to the large cover glass, and then placed in an oven at 37 °C for 1-2 h to accelerate the curing. 3.Other samples : Other samples can be operated with reference to the above samples.Matters needing attention：1. the anti fluorescence quenching blocker has poor anti quenching effect on staining live cell membrane dyes and mitochondrial dyes. It is recommended that the anti fluorescence quenching blocker be used to fix permeabilized cells or tissues. 2. anti fluorescence quenching tablet sealer and our YF ® 488 when matching dyes, background interference may occur, which may be caused by adding too much of this product. The amount of this product should be minimized. 3. the anti fluorescence quenching chip sealer may not be suitable for some dyes, so it is recommended to conduct pre experiment to test the matching. 4. during the film sealing process, avoid places with too much moisture. If the anti fluorescence quenching film sealing agent absorbs too much moisture, it will affect the film sealing effect. 5. for your safety and health, please wear experimental clothes and disposable gloves.Scope of application：Antifluorescent quencher... Read More | Copper tripeptide (GHK-Cu) is a naturally occurring tripeptide that is first isolated from human plasma but can also be found in saliva and urine. During wound healing, Copper tripeptide can be removed from existing extracellular proteins by protein hydrolysis and used as a chemical lure for Copper tripeptide (GHK-Cu) is a naturally occurring tripeptide that is first isolated from human plasma but can also be found in saliva and urine. During wound healing, Copper tripeptide can be removed from existing extracellular proteins by protein hydrolysis and used as a chemical lure for inflammatory and endothelial cells. Copper tripeptide can increase the production of messenger RNA in collagen, elastin, protein polysaccharides and glycosamine polysaccharides in fibroblasts. Copper tripeptide is a natural regulator of many cellular pathways in skin regeneration... Read More | Inquire | Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. 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