| Description | The anti fluorescence quenching sealing agent is a kind of reagent that can slow down the fluorescence quenching and cure the glass slide to form a permanent hard seal. The cured glass slide does not need to use nail polish or other sealant to seal the edge of the cover glass slide; DAPI containing The anti fluorescence quenching sealing agent is a kind of reagent that can slow down the fluorescence quenching and cure the glass slide to form a permanent hard seal. The cured glass slide does not need to use nail polish or other sealant to seal the edge of the cover glass slide; DAPI containing antifluorescent quench blocking agent can directly stain nuclei.Instruction：Note : Before use, return it to room temperature and mix well with slight oscillation to avoid bubbles. 1.Cell samples : ( 1 ) After dyeing, absorb the liquid ; ( 2 ) Add 10-20 µL of sealing agent to each glass slide, cover the cover glass with cells, so that the cells can fully contact the sealing agent, and slowly cover the cover glass to seal the operation. The tablets dizzy naturally, try not to have bubbles ; ( 3 ) Fluorescence microscope observation ; ( 4 ) After the end of the observation, placed at 4 °C or room temperature, overnight sealing, pay attention to avoid light. Note : In order to prevent the formation of bubbles, the sealing dose can be appropriately increased. For example, 100-200 µL can be added dropwise to the large cover glass, and then placed in an oven at 37 °C for 1-2 h to accelerate the curing.2.Tissue sections : ( 1 ) After the staining is completed, the staining solution is sucked off ; ( 2 ) Dropping 10-20 µL of anti-fluorescence quenching sealing agent on the tissue section, covering the cover glass to allow the tissue to fully contact the sealing agent, and slowly covering the cover glass during operation to make the sealing agent naturally dizzy, try not to have bubbles ; ( 3 ) Observation of tissue sections by fluorescence microscope ; ( 4 ) After the end of the observation, placed at 4 °C or room temperature, overnight sealing, pay attention to avoid light. Note : In order to prevent the formation of bubbles, the sealing dose can be appropriately increased. For example, 100-200 µL can be added dropwise to the large cover glass, and then placed in an oven at 37 °C for 1-2 h to accelerate the curing. 3.Other samples : Other samples can be operated with reference to the above samples.Matters needing attention：1. the anti fluorescence quenching blocker has poor anti quenching effect on staining live cell membrane dyes and mitochondrial dyes. It is recommended that the anti fluorescence quenching blocker be used to fix permeabilized cells or tissues. 2. anti fluorescence quenching tablet sealer and our YF ® 488 when matching dyes, background interference may occur, which may be caused by adding too much of this product. The amount of this product should be minimized. 3. the anti fluorescence quenching chip sealer may not be suitable for some dyes, so it is recommended to conduct pre experiment to test the matching. 4. during the film sealing process, avoid places with too much moisture. If the anti fluorescence quenching film sealing agent absorbs too much moisture, it will affect the film sealing effect. 5. for your safety and health, please wear experimental clothes and disposable gloves.Scope of application：Antifluorescent quencher... Read More | Nucleoprotein (396-404) TFA is the 396 to 404 fragment of lymphocytic choriomeningitis virus (LCMV). Nucleoprotein (396-404) TFA is the H-2D(b)-restricted immunodominant epitope and can be used as a molecular model of viral antigen.Biological Activity:Nucleoprotein (396-404) TFA is the 396 to 404 Nucleoprotein (396-404) TFA is the 396 to 404 fragment of lymphocytic choriomeningitis virus (LCMV). Nucleoprotein (396-404) TFA is the H-2D(b)-restricted immunodominant epitope and can be used as a molecular model of viral antigen.Biological Activity:Nucleoprotein (396-404) TFA is the 396 to 404 fragment of lymphocytic choriomeningitis virus (LCMV). Nucleoprotein (396-404) TFA is the H-2D(b)-restricted immunodominant epitope and can be used as a molecular model of viral antigen... Read More | Inquire | Purity>97% SDS-PAGE and HPLC analyses. FunctionLA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen Purity>97% SDS-PAGE and HPLC analyses. FunctionLA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.Post-translationalProteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)). Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor. NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils. NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets... Read More | Purity>95% SDS-PAGE.FunctionProbable cell adhesion protein |