| Description | ApplicationIt is used for the development and mass formulation of β-hydroxybutyrate reagents.Enzymatic propertiesSource: MicroorganismEnzymology Committee Number: EC1.1.1.30Molecular weight: 27 kDa (SDS-PAGE)Isoelectric point: 6.1Km value: 1.46×10-2M (D-3-Hydroxybutyrate), 9.5×10-5M (ApplicationIt is used for the development and mass formulation of β-hydroxybutyrate reagents.Enzymatic propertiesSource: MicroorganismEnzymology Committee Number: EC1.1.1.30Molecular weight: 27 kDa (SDS-PAGE)Isoelectric point: 6.1Km value: 1.46×10-2M (D-3-Hydroxybutyrate), 9.5×10-5M (NAD+)Inhibitor: Hg²⁺，Ag⁺，SDS Optimal pH: 8.5 Figure 1Optimum temperature: 60℃ Figure 2pH stability: 5.0-10.0 (25℃,16h) Figure 3Thermal stability: Stable below 50℃ (pH8.5, 30min) Figure 4Stability: -25 ~ -15℃ standing store for 12 months. More than 90% activity Figure 5 Assay method for activity1. PrincipleThe amount of NADH produced by the reaction can be measured by spectrophotometer at 340nm.2. Definition of enzyme activityUnit enzyme activity is defined as the amount of enzyme required to produce 1µmol of NADH per minute under the following reaction conditions.3. Reagent preparationReagent I: 100mM pH8.5 Tris-HCl buffer.Reagent II: 158mM sodium 3-hydroxybutyrate solution (dissolved with reagent I).Reagent III: 27.9mM NAD+ (dissolved with reagent I).Reagent IV: 100mM Tris-HCl, pH8.5, containing 0.1%BSA.Sample to be tested: Dilute the enzyme solution with reagent IV to 0.1-0.5 U/mL.4. Operation procedure4.1 Add 3mL reagent I, 0.5mL reagent II and 0.2mL reagent III into a 3mL colorimetric dish and preheat it at 37°C for 5min.4.2 Add 100µL sample to be tested and mix well.4.3 Reaction at 340nm at 37°C, record absorbance change within 1min (∆As)* Replace enzyme liquid with enzyme diluent, other steps are the same, the absorbance of the resulting solution is blank absorbance (∆Ab)∆A=∆As-∆Ab5. Vitality computingThe formula can be captured or edited in this positionVt: total volume of reaction liquid (3.1mL);Vs: enzyme liquid volume (0.1mL);1.0: optical path length (cm);df: dilution ratio;C: Enzyme concentration (mg/mL);6.22: Under standard reaction conditions, the millimolar absorption coefficient (cm2/µmol) of the color-producing group at 340nm... Read More | Purity: >90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.Epitope tagging offers an easy and universalPurity: >90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product... Read More | Purity≥ 92% SDS-PAGEActual molecular weight 15&17kDaFunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like Purity≥ 92% SDS-PAGEActual molecular weight 15&17kDaFunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis... Read More | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue StainingDescription:NG2, also known as CSPG4, MCSP, and AN2, is a 400-500 kDa transmembrane chondroitin sulfate proteoglycan (CSPG) with a protein core of approximately 300 kDa. The extracellular region can be proteolytically shed fromPurity:>95%, by SDS-PAGE visualized with Coomassie® Blue StainingDescription:NG2, also known as CSPG4, MCSP, and AN2, is a 400-500 kDa transmembrane chondroitin sulfate proteoglycan (CSPG) with a protein core of approximately 300 kDa. The extracellular region can be proteolytically shed from the cell surface. Mature human NG2 consists of a 2195 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain. Within aa 1583-2224, human NG2/CSPG4 shares 83% aa sequence identity with mouse and rat CSPG4. NG2 binds to the extracellular matrix proteins Laminin, Tenascin, and Collagens II, V, and VI as well as to the growth factors FGF-2 and PDGF-AA. NG2 is expressed on glial cell progenitors known as O2A cells or NG2 glia. These cells are neuronally responsive and differentiate primarily into oligodendrocytes but also into astrocytes. NG2 associates with PDGF R alpha and the AMPA R subunit GluR2. It is up-regulated on microglial cells during inflammation and contributes to the induction of inflammatory mediators. Various CSPGs in the brain inhibit neurite outgrowth through interactions with Nogo Receptor/NgR1 and NgR3. This recombinant protein product corresponds to the last 5 CSPG repeats, a region which can independently inhibit neurite outgrowth. NG2 is also expressed on vascular mural cells and capillaries. It promotes vascular endothelial cell (EC) migration and angiogenesis through interactions with Galectin-3 and Integrin alpha 3 beta 1 on EC, Plasminogen, and Angiostatin. NG2 is also expressed on a variety of tumors where it contributes to tumor cell adhesion, motility, and invasion... Read More | Inquire |