| Description | Hydrolyzed creatinine, used in the development and bulk formulation of enzymatic creatinine reagents.Enzymatic propertiesSource: MicroorganismEnzyme Committee No. : EC 3.5.2.10Molecular weight: 29 kDa (SDS-PAGE)Isoelectric point: 5.3Km value: 5.0× 10-2 M (Creatinine),8.0× 10-2 M (Creatine)Hydrolyzed creatinine, used in the development and bulk formulation of enzymatic creatinine reagents.Enzymatic propertiesSource: MicroorganismEnzyme Committee No. : EC 3.5.2.10Molecular weight: 29 kDa (SDS-PAGE)Isoelectric point: 5.3Km value: 5.0× 10-2 M (Creatinine),8.0× 10-2 M (Creatine)Inhibitors: Hg2+, Cu2+, Fe3+Optimal pH: 7.0-8.0 Figure 1 Optimum temperature: 65℃ Figure 2pH stability: pH 5.5-10.0 (25℃, 16 h) Figure 3Thermal stability: stable below 65℃ (pH 8.0, 30 min) Figure 4Stability: -25 ~ -15℃ standing storageMaintain over 90% activity for 12 months Figure 5 Assay method for activity1. Principle 2. Definition of enzyme activityUnit enzyme activity is defined as the amount of enzyme required to catalyze a reaction to produce 1µmol creatine per minute under the following conditions.3. Reagent preparationReagent I: 0.3M potassium phosphate buffer, pH 6.5.Reagent II: 0.1M creatinine solution (1.13g creatinine dissolved in 100mL UP water).Reagent III: 4% Na2CO3 solution (4.0g Na2CO3 dissolved in 100mL UP water).Reagent IV: 2% alpha-naphthol solution (2.0g alpha-naphthol dissolved in 100 mL of 99.5% ethanol).Reagent V: 1.2g NaOH and 3.2g Na2CO3 were dissolved in double steaming water at a constant volume of 100 mL.Reagent VI: 0.05% diacetyl solution (0.05mL diacetyl with water to 100 mL).Enzyme diluent: 5 mM Tris-HCl pH 8.04. Operation procedure4.1. Add 0.1 mL reagent I and 0.8mL reagent II into a 5 mL centrifuge tube.4.2. Water bath at 37℃ for 5 minutes.4.3. Add 0.1 mL of the sample to be tested and incubate at 37℃ for 10 minutes.4.4. Add 2.0mL reagent III to terminate the reaction, remove and place on ice.4.5. Add the following reagents in the new 5 mL centrifuge tube in order:The solution for step 4: 80 µLDouble steaming water: 720 µLReagent IV: 400 µLReagent V: 400 µLReagent VI: 400 µL4.6. After standing at 25℃ for 1 h, add 2 mL of double steaming water to dilute.4.7. Use a spectrophotometer to measure the absorbance at 525 nm.* Replace enzyme liquid with enzyme diluent, other steps are the same, the absorbance of the resulting solution is blank absorbance (∆Ab)∆A=∆As- ∆Ab5. Vitality computing Vt: Total volume of reaction liquid (1.0mL);Vs: Enzyme liquid volume (0.1mL);t: Reaction time (10 minutes);df: Dilution ratio;C: Enzyme concentration (mg/mL);1.0: Optical path length (cm);0.0704: Millimolar absorption coefficient (cm²/µmol) of chromophore at 525nm under standard reaction conditions... Read More | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG) (By similarity).Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG) (By similarity).Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger. Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation (By similarity).Fc-binding subunit that associates with FCER1G adapters to form functional signaling complexes. Following the engagement of antigen-IgG complexes, triggers phosphorylation of immunoreceptor tyrosine-based activation motif (ITAM)-containing adapters with subsequent activation of phosphatidylinositol 3-kinase signaling and sustained elevation of intracellular calcium that ultimately drive NK cell activation (By similarity).Mediates enhanced ADCC in response to afucosylated IgGs... Read More | Purity>95% as determined by SDS-PAGE and Coomassie blue stainingFunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.Sequence Purity>95% as determined by SDS-PAGE and Coomassie blue stainingFunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.Sequence similaritiesBelongs to the heparin-binding growth factors family.Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1... Read More | Purity>97% SDS-PAGE.FunctionReceptor for interleukin-2 | Inquire |