| Description | The 6X His Peptide is commonly used for the competitive elution of His tag fusion proteins bound to His antibodies. It can be used to elute His tag fusion proteins from Anti-His Magnetic Beads and conventional Anti-His antibodies during immunoprecipitation.This product competitively elutes the 6X The 6X His Peptide is commonly used for the competitive elution of His tag fusion proteins bound to His antibodies. It can be used to elute His tag fusion proteins from Anti-His Magnetic Beads and conventional Anti-His antibodies during immunoprecipitation.This product competitively elutes the 6X His fusion protein. The eluted protein sample does not contain the light and heavy chains of Anti-His antibody, thus effectively eliminating the interference of anibodies with downstream Western blot analysis.The amino acid sequence of 6X His Peptide is His-His-His-His-His-His (HHHHHH).The main parameters of 6X His Peptide are as followsPrecautions：The solution of this product should be aliquoted and stored at -20℃ or lower temperatures to avoid repeated freeze-thaw cycles.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：1. For 25mg provided in powder form, centrifuge at 8,000-12,000×g for 10-30 seconds before opening to collect the powder at the bottom of the tube. Dissolve it in TBS by gently pipetting or shaking. Do not vortex vigorously to avoid denaturation and inactivation of the peptide.2. P9811-1mg and P9811-5mg can be directly used for preparing elution buffer. 3. Competitive elution with the 6X His Peptide: This elution method is non-denaturing, with high elution efficiency, and the eluted sample does not contain the light and heavy chains of His antibodies.a. Preparation of 6X His tag elution buffer: Based on the required amount of elution buffer, dilute an appropriate amount of 6X His Peptide stock solution with TBS to a final concentration of 150µg/ml.b. Add 100µl of 6X His tag elution buffer (150µg/ml) to each immunoprecipitated sample, and incubate on ice or at 4℃ for 30 minutes to 2 hours with shaking. In order to improve the elution efficiency, the incubation time can be extended or the elution can be repeated. The volume of the 6X His tag elution buffer is generally 5 times that of the bead or gel suspension.c. Centrifuge at 6000×g for 30 seconds at 4℃, and carefully transfer the supernatant to a new tube. The supernatant contains the 6X His fusion protein and its protein complexes. Be careful not to touch the resin when taking the supernatant. 4. Store the eluted 6X His fusion protein at 4℃ for immediate use, or at -20℃ for long-term storage... Read More | Product Application：KNK437 has been used: as a heat shock factor 1 (HSF1) inhibitor to study its effects on the inhibition of viability and apoptosis activation in chemoresistant mice cells as an HSF1 inhibitor to study its effects on viability and apoptosis of colorectal cancer cells as a Product Application：KNK437 has been used: as a heat shock factor 1 (HSF1) inhibitor to study its effects on the inhibition of viability and apoptosis activation in chemoresistant mice cells as an HSF1 inhibitor to study its effects on viability and apoptosis of colorectal cancer cells as a heat shock protein 70 (HSP70) inhibitor to study its effects on glutamine-induced HSP70 and inflammatory mediator release... Read More | Inquire | Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleavesRibonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies. ApplicationRibonuclease T1 has extensive applications in molecular cloning and DNA sequencing. Because of its specificity it has been a commonly used cleavage enzyme for the determination of structure, nearest neighbor frequencies, and RNA sequencing. The enzyme has further application in the preparation of nucleoside 2',3'-cyclic phosphates, the synthesis of oligonucleotides, and the removal of RNA from DNA preparations. The enzyme is also used as a non-mammalian source of RNase in various applications... Read More | Product Characteristics UNI-StabilPLUS is a universal stabilizer for the dilution and stabilization of both Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP) labeled proteins and antibodies, in order to maintain the molecular conformation and prevent loss of activity over time. This enablesProduct Characteristics UNI-StabilPLUS is a universal stabilizer for the dilution and stabilization of both Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP) labeled proteins and antibodies, in order to maintain the molecular conformation and prevent loss of activity over time. This enables the making of pre-diluted, ready-to-use conjugates, minimizing assay errors in dilution. Superior stabilization of HRP and AP conjugated antibodies in low as well as high protein dilutions is seen, when using UNI-StabilPLUS. When tested with AP conjugated antibody stability is seen as follows: • at least 3 years at 2-8 °C • at least 2 years at room temperature • at least 4 weeks at 37 °C When tested with HRP conjugated antibody stability is seen as follows: • at least 2 years at 2-8 °C • at least 1 years at room temperature • at least 2 weeks at 37 °CUNI-StabilPLUS is recommended for the dilution of antibodies directed against rabbit immunoglobulins unlike HRP-StabilPLUS (cat. no. H494387) and Antibody Enhancer (cat. no. A494276).Composition & Properties UNI-StabilPLUS is a ready-to use buffer that appears as an opaque solution. The product is based on a mild acid Tris buffer containing proprietary stabilizing components. UNI-StabilPLUS contains neither BSA, nor other material from bovine serum, no azide, mercury or other toxic components.Working Procedure 1.Make a series of dilutions of the HRP- or AP conjugated protein in UNI-StabilPLUS in order to determine the optimal dilution. 2.Run the assay as usual or store the diluted conjugated protein preferably at 2-8 °C.Tips & Tricks • Avoid using phosphate buffers for AP-conjugated antibody assays. We recommend the use of Tris/HCl, Tween as the washing buffer, instead of a PBS buffer which will reduce signal significantly. • For extended stability of HRP conjugated antibodies, HRP-StabilPLUS (cat. no. H494387) is recommended. Handling & Storage • Store solution at 2-8 °C... Read More |