| Description | UltraBio™ Multiple Amplification Mix is a universal multiplex amplification reagent that can be used for constructing multiplex amplicon libraries for microbial/genetic mutation detection. It is compatible with ultra-multiplex amplification, with a primer number range of 1 to 1500, amplicon UltraBio™ Multiple Amplification Mix is a universal multiplex amplification reagent that can be used for constructing multiplex amplicon libraries for microbial/genetic mutation detection. It is compatible with ultra-multiplex amplification, with a primer number range of 1 to 1500, amplicon length range of 100 to 1800 bp, GC content range of 13% to 75%, and a minimum template input amount of 1 ng. The reagent undergoes strict background microbe quality control to minimize false positives from non-specific amplification. Rigorous functional validation ensures high stability and reproducibility, making it ideal for NGS-based multiplex targeted amplification in microbial/genetic mutation detection.Self-Supplied Materials1. Instruments PCR thermal cycler, Pipettes, Magnetic stand, Microcentrifuge, Vortex mixer, Agarose gel electrophoresis system, UV gel imaging system, Agilent Bioanalyzer 2100 (or equivalent).2. Reagents Agarose, 1× TAE buffer, Nucleic acid dye, DNA marker, Ampure XP beads (or equivalent), Freshly prepared 80% ethanol, Nuclease-free water.3. Consumables 1.5 mL microcentrifuge tubes, 0.2 mL PCR tubes, DNase/RNase-free pipette tips.ProtocolI. Multiplex Amplification & Product VerificationReagent PreparationThaw UltraBio™ Multiplex Amplification Mix on ice.Vortex briefly and centrifuge before use.Reaction Setup (10 µL Standard Volume)ComponentVolume per ReactionFinal ConcentrationUltraBio™ Multiplex Amplification mix2.5 µL4xPrimer Mix1 µL20nM (2.5~100nM)Template DNAX µL10ng (1~100ng)Nuclease-free waterUp to10 µL/Total10 µL/Note:Total reaction volume can be scaled to 10–50 µL (adjust mix proportionally).Primer concentration and annealing temperature (T) should be optimized for each panel.Mixing & CentrifugationVortex gently and centrifuge briefly to collect liquid.PCR Cycling Program*T: Annealing temperature depends on primer panel (optimize empirically).Cycle number: Adjust based on template input (default: 25 cycles).Product QC: Analyze amplification success via 2% agarose gel electrophoresis or Agilent 2100 Bioanalyzer.II. Amplicon Purification (Ampure XP Beads)Bead EquilibrationWarm Ampure XP beads to room temperature; vortex thoroughly.Bead BindingDilute PCR products to 50 µL with nuclease-free water.Add 60 µL beads (1.2× ratio), vortex, and incubate 5 min at RT.Wash StepsPlace tubes on a magnetic stand for 3–5 min; discard supernatant.Wash twice with 200 µL fresh 80% ethanol (30 sec per wash).Air-dry beads for 2–3 min until matte.ElutionResuspend beads in 22 µL nuclease-free water; incubate 2 min at RT.Transfer 20 µL supernatant to a new PCR tube. Store purified products at –20°C... Read More | Inquire | EPOCROSTM is a reactive polymer with an oxazoline group on the side chain and is used as a cross-linking agent for water-based resins. Among the water-based polymers developed to address environmental issues and the increasing use of water-based products due to VOC regulations and desolventing, the EPOCROSTM is a reactive polymer with an oxazoline group on the side chain and is used as a cross-linking agent for water-based resins. Among the water-based polymers developed to address environmental issues and the increasing use of water-based products due to VOC regulations and desolventing, the EPOCROSTM WS series is a “water-soluble type” with the following structure.Features and PropertiesHigher reactivity than water-based epoxy, melamine, blocked isocyanateVOC free (EPOCROS™ WS-300 and EPOCROS™ WS-700)High crosslinking density with a small amount addedOne-pack type with long usage timeImproves water resistance, solvent resistance, heat resistance, and the strength of films, etc.Adhesion-imparting possible to PET, OPP, PVC, etc.Fast dryingLow toxicity (Ames Test: Negative, Primary Skin Irritation Test: No irritation)WS Series Product LineupApplicationsNonwoven fabric bindersPigment printingCoatings (metals, films, leather)Paint and coatings, Primers (plastics, building materials, vehicles)AdhesivesMethodASSAY for Product Code DILW:One unit equals a decrease in absorbance of 1.0 per minute at 25°C at pH 7.5 with 2,6-dichlorophenolindophenol as the chromogen.Reagents0.2 M Tris⋅HCl buffer, pH 7.50.006 M NADH. Prepare fresh daily.0.0012 M Dichlorophenolindophenol (DCPIP) Prepare fresh daily.EnzymePrepare a 10 mg/ml solution of enzyme in 0.2 M Tris⋅HCl, pH 7.5.Dilute further immediately before use to give ΔA/min of 0.15-0.20.ProcedureAdjust spectrophotometer to 600 nm and 25°C.Pipette into cuvettes as follows:Mix quickly and measure the decrease in absorbance at 600 nm for 2-3 minutes.Determine the ΔA/min. from the initial linear portion of the curve. (Use portion of curve from t=0 to t=1 minute; the rate is linear for 1/2 to 1 minute.)Calculation... Read More | Inquire | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： 100B, previously called S100 beta, belongs to the S100 family within the EF-hand superfamily of Ca2+ binding proteins. S100 proteins contain two EF-hand motifs that differ in affinity, separated by a hingePurity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： 100B, previously called S100 beta, belongs to the S100 family within the EF-hand superfamily of Ca2+ binding proteins. S100 proteins contain two EF-hand motifs that differ in affinity, separated by a hinge region with a hydrophobic cleft that is exposed upon Ca2+ binding. S100B is a 91 amino acid (aa) protein, after removal of the initial methionine, and is found as homodimers of 10.4 kDa monomers. Human S100B shares 99%, 98%, 100%, 99% and 97% aa sequence identity with mouse, rat, rabbit, equine and bovine S100B, respectively. Within the S100 family, human S100B shows the highest aa identity (59%) with S100A1. S100B is expressed primarily by astrocytes and oligodendrocytes in the central nervous system, and by Schwann cells in the peripheral nervous system. Ca2+-bound S100B interacts in vitro with at least 20 cytoplasmic proteins, including several structural molecules such as tubulin and GFAP. It can inhibit the phosphorylation of these kinase substrates and others such as tau and neuromodulin. Astrocytes can secrete S100B, which then acts in a cytokine-like manner. Nanomolar concentrations of S100B are secreted constitutively, promote proliferation, and are neurotrophic and anti-apoptotic. Blood levels of S100B reflect extracellular concentrations within the nervous system, and are elevated in Down’s syndrome, Alzheimer’s disease and Tourette’s syndrome, metabolic stress, acute brain injury and brain tumors. Micromolar concentrations of S100B can be destructive and pro-apoptotic; they induce the expression of iNOS, COX-2, IL-1, IL‑6 and TNF-alpha by microglia, astrocytes or neurons. Most extracellular actions of S100B can be mediated by RAGE (receptor for advanced glycation end products), which is also a receptor for other S100 proteins... Read More |