| Description | Esterase, also known as carboxyl ester hydrolases, are a member of the hydrolytic enzyme superfamily. Esterase is widely present in organisms, such as helping to digest esters in food in the digestive system, and also participating in biological processes such as fatty acid metabolism within cells. Esterase, also known as carboxyl ester hydrolases, are a member of the hydrolytic enzyme superfamily. Esterase is widely present in organisms, such as helping to digest esters in food in the digestive system, and also participating in biological processes such as fatty acid metabolism within cells. Esterase is widely used in industry, such as in the production of biodiesel, food additives, and cosmetics. ≥15U /mg powder, ≥100 U/mg protein. Source: Porcine liver.Properties The molecular weight of esterase monomer is approximately 66kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half molecules in the presence of dilute acid or concentrated saltStabilityEsterase acts on water-soluble carboxylic acid esters containing short chain fatty acids. Its function comes from the catalytic triad of Ser, His, and Asp/Glu, which is stable in the form of a triad under low temperature and low pH conditions.Application1、Used for kinetic resolution and asymmetric synthesis in organic chemistry.2、Used to reveal fluorescence in the UV visible spectrum.3、Used in pharmaceutical and industrial applications to catalyze the hydrolysis of pentaacetyl catechins and epicatechin.4、It is used to develop an alternative experimental scheme based on gel for screening bead binding catalytic activity in aqueous media.Attention1、 Avoid contact with skin and eyes.2、 For your safety and health, please wear Lab coat and disposable gloves... Read More | Inquire | p53 and MDM2 proteins-interaction-inhibitor dihydrochloride is an inhibitor of the interaction between p53 and MDM2 proteins.Form:Solid | Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. IL12 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Recombinant human IL12 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques... Read More | BackgroundStreptavidin is a tetrameric bacterial protein isolated from Streptomyces avidinii providing 4 high-affinity biotin binding sites. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin. With a dissociation constant on the order of ≈10⁻¹⁴ mol/L,BackgroundStreptavidin is a tetrameric bacterial protein isolated from Streptomyces avidinii providing 4 high-affinity biotin binding sites. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin. With a dissociation constant on the order of ≈10⁻¹⁴ mol/L, the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Unlike egg-white avidin, which has a net positive charge at neutral pH and contains about 7% carbohydrate, streptavidin has almost no net charge at neutral pH, does not contain carbohydrate, and exhibits lower non-specific background. Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules. This FITC-streptavidin conjugate was prepared by highly purified Streptavidin and free FITC was removed. Streptavidin (FITC) is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. Excitation at 488nm light leads to a fluorescence emission maximum of 520 nm.Recommended Usage：Every lot of Streptavidin-FITC is tested by flow cytometry using biotinylated primary antibodies. From this testing it is recommended that between 0.02 and 0.25 µg of streptavidin be used per 106 cells in a 100 µl staining volume... Read More |